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BCLAF1 Antibody (C-term)
Affinity Purified Rabbit Polyclonal Antibody (Pab)
- SPECIFICATION
- CITATIONS
- PROTOCOLS
- BACKGROUND
Application 
| WB, IHC-P, E |
|---|---|
| Primary Accession | Q9NYF8 |
| Other Accession | NP_055554.1, NP_001070908.1, NP_001070909.1 |
| Reactivity | Human |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | Rabbit IgG |
| Calculated MW | 106122 Da |
| Antigen Region | 658-687 aa |
| Gene ID | 9774 |
|---|---|
| Other Names | Bcl-2-associated transcription factor 1, Btf, BCLAF1, BTF, KIAA0164 |
| Target/Specificity | This BCLAF1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 658-687 amino acids from the C-terminal region of human BCLAF1. |
| Dilution | WB~~1:1000 IHC-P~~1:50~100 E~~Use at an assay dependent concentration. |
| Format | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
| Precautions | BCLAF1 Antibody (C-term) is for research use only and not for use in diagnostic or therapeutic procedures. |
| Name | BCLAF1 |
|---|---|
| Synonyms | BTF, KIAA0164 |
| Function | Death-promoting transcriptional repressor. May be involved in cyclin-D1/CCND1 mRNA stability through the SNARP complex which associates with both the 3'end of the CCND1 gene and its mRNA. |
| Cellular Location | Cytoplasm. Nucleus. Nucleus speckle. Nucleus, nucleoplasm |
| Tissue Location | Ubiquitous. |
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Provided below are standard protocols that you may find useful for product applications.
Background
This gene encodes a transcriptional repressor that interacts with several members of the BCL2 family of proteins. Overexpression of this protein induces apoptosis, which can be suppressed by co-expression of BCL2 proteins. The protein localizes to dot-like structures throughout the nucleus, and redistributes to a zone near the nuclear envelope in cells undergoing apoptosis. Multiple transcript variants encoding different isoforms have been found for this gene.
References
Sarras, H., et al. ScientificWorldJournal 10, 1450-1461 (2010) :
Renert, A.F., et al. J. Proteome Res. 8(10):4810-4822(2009)
Liu, H., et al. Mol. Cell. Biol. 27(24):8480-8491(2007)
Sugiyama, N., et al. Mol. Cell Proteomics 6(6):1103-1109(2007)
Olsen, J.V., et al. Cell 127(3):635-648(2006)
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