USP50 Antibody (Center)
Affinity Purified Rabbit Polyclonal Antibody (Pab)
- SPECIFICATION
- CITATIONS
- PROTOCOLS
- BACKGROUND
Application
| IHC-P, WB, E |
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Primary Accession | Q70EL3 |
Other Accession | NP_987090.1 |
Reactivity | Human |
Host | Rabbit |
Clonality | Polyclonal |
Isotype | Rabbit IgG |
Calculated MW | 38955 Da |
Antigen Region | 141-169 aa |
Gene ID | 373509 |
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Other Names | Inactive ubiquitin carboxyl-terminal hydrolase 50, Inactive ubiquitin-specific peptidase 50, USP50 |
Target/Specificity | This USP50 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 141-169 amino acids from the Central region of human USP50. |
Dilution | WB~~1:1000 IHC-P~~1:50~100 |
Format | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
Precautions | USP50 Antibody (Center) is for research use only and not for use in diagnostic or therapeutic procedures. |
Name | USP50 (HGNC:20079) |
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Function | Deubiquitinating enzyme that removes conjugated ubiquitin from specific proteins to regulate different cellular processes. Regulates the inflammasome signaling pathway by deubiquitinating 'Lys- 63'-linked polyubiquitination of the PYCARD/ASC adapter protein (PubMed:28094437). Regulates the ubiquitination and stability of the ACE2 protein (PubMed:36876523). Acts as a negative regulator of the G2/M checkpoint pathway, by preventing serine/threonine kinase WEE1 degradation, thereby repressing entry into mitosis following activation of the G2/M DNA damage checkpoint (PubMed:20930503). |
Cellular Location | Cytoplasm. Cytoplasm, cytoskeleton, microtubule organizing center, centrosome. Nucleus Note=Accumulates in the nucleus following DNA injury |
Tissue Location | Weakly expressed in a few tissues. |
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Provided below are standard protocols that you may find useful for product applications.
Background
Has no peptidase activity.
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