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WDR70 Antibody (C-term)

Affinity Purified Rabbit Polyclonal Antibody (Pab)

     
  • WB - WDR70 Antibody (C-term) AP11355B
    Anti-WDR70 Antibody (C-term) at 1:1000 dilution + HL-60 whole cell lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 70 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
    detail
  • IF - WDR70 Antibody (C-term) AP11355B
    Confocal immunofluorescent analysis of WDR70 Antibody (C-term)(Cat#AP11355b) with Hela cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).
    detail
  • WB - WDR70 Antibody (C-term) AP11355B
    WDR70 Antibody (C-term) (Cat. #AP11355b) western blot analysis in Hela cell line lysates (35ug/lane).This demonstrates the WDR70 antibody detected the WDR70 protein (arrow).
    detail
  • IHC-P - WDR70 Antibody (C-term) AP11355B
    WDR70 Antibody (C-term) (Cat. #AP11355b)immunohistochemistry analysis in formalin fixed and paraffin embedded human esophageal carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of WDR70 Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.
    detail
  • FC - WDR70 Antibody (C-term) AP11355B
    WDR70 Antibody (C-term) (Cat. #AP11355b) flow cytometric analysis of Hela cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
    detail
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
WB, FC, IF, IHC-P, E
Primary Accession Q9NW82
Other Accession Q5EB92, Q3TWF6, Q32LB0, NP_060504.1
Reactivity Human
Predicted Bovine, Mouse, Rat
Host Rabbit
Clonality Polyclonal
Isotype Rabbit IgG
Calculated MW 73201 Da
Antigen Region 575-604 aa
Additional Information
Gene ID 55100
Other Names WD repeat-containing protein 70, WDR70
Target/Specificity This WDR70 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 575-604 amino acids from the C-terminal region of human WDR70.
Dilution WB~~1:1000
IF~~1:10~50
IHC-P~~1:50~100
FC~~1:10~50
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsWDR70 Antibody (C-term) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name WDR70
Research Areas
Citations (0)
citation

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References

Olsen, J.V., et al. Cell 127(3):635-648(2006)
Ota, T., et al. Nat. Genet. 36(1):40-45(2004)

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$ 365.00
$ 140.00
Cat# AP11355B
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