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UGT8 antibody (Center)
Affinity Purified Rabbit Polyclonal Antibody (Pab)
- SPECIFICATION
- CITATIONS: 1
- PROTOCOLS
- BACKGROUND
Application 
| WB, IHC-P, E |
|---|---|
| Primary Accession | Q16880 |
| Other Accession | Q09426, Q64676, NP_001121646.1, NP_003351.2 |
| Reactivity | Human, Mouse |
| Predicted | Rat |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | Rabbit IgG |
| Calculated MW | 61438 Da |
| Antigen Region | 366-393 aa |
| Gene ID | 7368 |
|---|---|
| Other Names | 2-hydroxyacylsphingosine 1-beta-galactosyltransferase, Ceramide UDP-galactosyltransferase, Cerebroside synthase, UDP-galactose-ceramide galactosyltransferase, UGT8, CGT, UGT4 |
| Target/Specificity | This UGT8 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 366-393 amino acids from the Central region of human UGT8. |
| Dilution | WB~~1:1000 IHC-P~~1:10~50 E~~Use at an assay dependent concentration. |
| Format | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
| Precautions | UGT8 antibody (Center) is for research use only and not for use in diagnostic or therapeutic procedures. |
| Name | UGT8 (HGNC:12555) |
|---|---|
| Synonyms | CGT, UGT4 |
| Function | Catalyzes the transfer of galactose to ceramide, a key enzymatic step in the biosynthesis of galactocerebrosides, which are abundant sphingolipids of the myelin membrane of the central nervous system and peripheral nervous system (PubMed:9125199). Galactosylates both hydroxy- and non-hydroxy fatty acid-containing ceramides and diglycerides (By similarity). |
| Cellular Location | Membrane; Single-pass membrane protein. Endoplasmic reticulum {ECO:0000250|UniProtKB:Q09426} |
Provided below are standard protocols that you may find useful for product applications.
Background
Galactocerebrosides are abundant sphingolipids of the myelin membrane of the central nervous system and peripheral nervous system and are also present in small amounts in kidney. The key enzymatic step in the biosynthesis of galactocerebrosides consists of the transfer of galactose to ceramide catalyzed by UDP-galactose ceramide galactosyltransferase (CGT, EC 2.4.1.45). The enzyme encoded by the CGT gene is the first involved in complex lipid biosynthesis in the myelinating oligodendrocyte.[supplied by OMIM].
References
Dziecedil Giel, P., et al. Br. J. Cancer 103(4):524-531(2010)
Kalsi, G., et al. Hum. Mol. Genet. 19(12):2497-2506(2010)
Ross, C.J., et al. Nat. Genet. 41(12):1345-1349(2009)
Ruckhaberle, E., et al. J. Cancer Res. Clin. Oncol. 135(8):1005-1013(2009)
Saito, A., et al. J. Hum. Genet. 54(6):317-323(2009)
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