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PCDH1 Antibody (N-term)

Affinity Purified Rabbit Polyclonal Antibody (Pab)

     
  • IF - PCDH1 Antibody (N-term) AP12019a
    Confocal immunofluorescent analysis of PCDH1 Antibody (N-term)(Cat#AP12019a) with U-251MG cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).
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  • WB - PCDH1 Antibody (N-term) AP12019a
    PCDH1 Antibody (N-term) (Cat. #AP12019a) western blot analysis in MDA-MB453,ZR-75-1 cell line lysates (35ug/lane).This demonstrates the PCDH1 antibody detected the PCDH1 protein (arrow).
    detail
  • IHC-P - PCDH1 Antibody (N-term) AP12019a
    PCDH1 Antibody (N-term) (Cat. #AP12019a)immunohistochemistry analysis in formalin fixed and paraffin embedded human brain tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of PCDH1 Antibody (N-term) for immunohistochemistry. Clinical relevance has not been evaluated.
    detail
  • SPECIFICATION
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
IF, WB, IHC-P, E
Primary Accession Q08174
Other Accession NP_115796.2
Reactivity Human
Host Rabbit
Clonality Polyclonal
Isotype Rabbit IgG
Calculated MW 114743 Da
Antigen Region 179-206 aa
Additional Information
Gene ID 5097
Other Names Protocadherin-1, Cadherin-like protein 1, Protocadherin-42, PC42, PCDH1
Target/Specificity This PCDH1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 179-206 amino acids from the N-terminal region of human PCDH1.
Dilution IF~~1:10~50
WB~~1:1000
IHC-P~~1:10~50
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsPCDH1 Antibody (N-term) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name PCDH1
Function May be involved in cell-cell interaction processes and in cell adhesion.
Cellular Location Cell junction. Cell membrane; Single-pass type I membrane protein. Note=Found at cell- cell boundaries and probably at cell-matrix boundaries
Tissue Location Highly expressed in the brain and neuro-glial cells
Research Areas
Citations (0)
citation

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Background

This gene belongs to the protocadherin subfamily within the cadherin superfamily. The encoded protein is a membrane protein found at cell-cell boundaries. It is involved in neural cell adhesion, suggesting a possible role in neuronal development. The protein includes an extracelllular region, containing 7 cadherin-like domains, a transmembrane region and a C-terminal cytoplasmic region. Cells expressing the protein showed cell aggregation activity. Alternative splicing occurs in this gene.

References

Koppelman, G.H., et al. Am. J. Respir. Crit. Care Med. 180(10):929-935(2009)
Rush, J., et al. Nat. Biotechnol. 23(1):94-101(2005)
Ballif, B.A., et al. Mol. Cell Proteomics 3(11):1093-1101(2004)
Ballif, B.A., et al. Mol. Cell Proteomics 3(11):1093-1101(2004)
Colland, F., et al. Genome Res. 14(7):1324-1332(2004)

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$ 365.00
$ 140.00
Cat# AP12019a
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