LRSAM1 Antibody (N-term)
Affinity Purified Rabbit Polyclonal Antibody (Pab)
- SPECIFICATION
- CITATIONS
- PROTOCOLS
- BACKGROUND
Application
| IHC-P, WB, E |
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Primary Accession | Q6UWE0 |
Other Accession | Q80ZI6, NP_612370.3, NP_001177652.1 |
Reactivity | Mouse |
Host | Rabbit |
Clonality | Polyclonal |
Isotype | Rabbit IgG |
Calculated MW | 83594 Da |
Antigen Region | 1-30 aa |
Gene ID | 90678 |
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Other Names | E3 ubiquitin-protein ligase LRSAM1, 632-, Leucine-rich repeat and sterile alpha motif-containing protein 1, Tsg101-associated ligase, hTAL, LRSAM1, TAL |
Target/Specificity | This LRSAM1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 1-30 amino acids from the N-terminal region of human LRSAM1. |
Dilution | WB~~1:1000 IHC-P~~1:10~50 |
Format | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
Precautions | LRSAM1 Antibody (N-term) is for research use only and not for use in diagnostic or therapeutic procedures. |
Name | LRSAM1 {ECO:0000303|PubMed:20865121} |
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Function | E3 ubiquitin-protein ligase that mediates monoubiquitination of TSG101 at multiple sites, leading to inactivate the ability of TSG101 to sort endocytic (EGF receptors) and exocytic (HIV-1 viral proteins) cargos (PubMed:15256501). Bacterial recognition protein that defends the cytoplasm from invasive pathogens (PubMed:23245322). Localizes to several intracellular bacterial pathogens and generates the bacteria-associated ubiquitin signal leading to autophagy-mediated intracellular bacteria degradation (xenophagy) (PubMed:23245322, PubMed:25484098). |
Cellular Location | Cytoplasm. Note=Displays a punctuate distribution and localizes to a submembranal ring (PubMed:15256501). Localizes to intracellular bacterial pathogens (PubMed:23245322) |
Tissue Location | Highly expressed in adult spinal cord motoneurons as well as in fetal spinal cord and muscle tissue |
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Provided below are standard protocols that you may find useful for product applications.
Background
LRSAM1 is a multifunctional RING finger protein that selectively regulates cell adhesion molecules, has ubiquitin ligase activity, and plays a role in receptor endocytosis and viral budding (Li et al., 2003 [PubMed 14635195]; Amit et al., 2004 [PubMed 15256501]).
References
Markson, G., et al. Genome Res. 19(10):1905-1911(2009)
van Wijk, S.J., et al. Mol. Syst. Biol. 5, 295 (2009) :
McDonald, B., et al. Mol. Biol. Cell 19(2):754-763(2008)
Amit, I., et al. Genes Dev. 18(14):1737-1752(2004)
Li, B., et al. J. Cell. Biochem. 90(6):1224-1241(2003)
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