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MEPCE Antibody (Center)
Affinity Purified Rabbit Polyclonal Antibody (Pab)
- SPECIFICATION
- CITATIONS
- PROTOCOLS
- BACKGROUND
Application 
| WB, E |
|---|---|
| Primary Accession | Q7L2J0 |
| Other Accession | NP_001181921.1, NP_001181920.1, NP_001181919.1, NP_062552.2 |
| Reactivity | Mouse |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | Rabbit IgG |
| Calculated MW | 74355 Da |
| Antigen Region | 239-267 aa |
| Gene ID | 56257 |
|---|---|
| Other Names | 7SK snRNA methylphosphate capping enzyme, MePCE, 211-, Bicoid-interacting protein 3 homolog, Bin3 homolog, MEPCE, BCDIN3 |
| Target/Specificity | This MEPCE antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 239-267 amino acids from the Central region of human MEPCE. |
| Dilution | WB~~1:1000 E~~Use at an assay dependent concentration. |
| Format | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
| Precautions | MEPCE Antibody (Center) is for research use only and not for use in diagnostic or therapeutic procedures. |
| Name | MEPCE {ECO:0000303|PubMed:30559425, ECO:0000312|HGNC:HGNC:20247} |
|---|---|
| Function | S-adenosyl-L-methionine-dependent methyltransferase that adds a methylphosphate cap at the 5'-end of 7SK snRNA (7SK RNA), leading to stabilize it (PubMed:17643375, PubMed:19906723, PubMed:30559425). Also has a non-enzymatic function as part of the 7SK RNP complex: the 7SK RNP complex sequesters the positive transcription elongation factor b (P-TEFb) in a large inactive 7SK RNP complex preventing RNA polymerase II phosphorylation and subsequent transcriptional elongation (PubMed:17643375). The 7SK RNP complex also promotes snRNA gene transcription by RNA polymerase II via interaction with the little elongation complex (LEC) (PubMed:28254838). In the 7SK RNP complex, MEPCE is required to stabilize 7SK RNA and facilitate the assembly of 7SK RNP complex (PubMed:19906723, PubMed:38100593). MEPCE has a non- enzymatic function in the 7SK RNP complex; interaction with LARP7 within the 7SK RNP complex occluding its catalytic center (PubMed:19906723). Also required for stability of U6 snRNAs (PubMed:38100593). |
| Cellular Location | Nucleus. |
| Tissue Location | Expressed in chronic myeloid leukemia cells, adrenal gland, brain, cerebellum, kidney, lung, mammary gland and testis (PubMed:12358911). Weakly or not expressed in other tissues (PubMed:12358911). |
Thousands of laboratories across the world have published research that depended on the performance of antibodies from Abcepta to advance their research. Check out links to articles that cite our products in major peer-reviewed journals, organized by research category.
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Provided below are standard protocols that you may find useful for product applications.
Background
S-adenosyl-L-methionine-dependent methyltransferase that adds a methylphosphate cap at the 5'-end of 7SK snRNA, leading to stabilize it.
References
Xue, Y., et al. Nucleic Acids Res. 38(2):360-369(2010)
Krueger, B.J., et al. Nucleic Acids Res. 36(7):2219-2229(2008)
Kaneko, S., et al. Proc. Natl. Acad. Sci. U.S.A. 104(45):17620-17625(2007)
Jeronimo, C., et al. Mol. Cell 27(2):262-274(2007)
Olsen, J.V., et al. Cell 127(3):635-648(2006)
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