Bik BH3 Domain Antibody
Purified Rabbit Polyclonal Antibody (Pab)
- SPECIFICATION
- CITATIONS: 1
- PROTOCOLS
- BACKGROUND
Application ![]()
| WB, IHC-P, E |
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Primary Accession | Q13323 |
Reactivity | Human |
Host | Rabbit |
Clonality | Polyclonal |
Isotype | Rabbit IgG |
Calculated MW | 18016 Da |
Antigen Region | 39-74 aa |
Gene ID | 638 |
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Other Names | Bcl-2-interacting killer, Apoptosis inducer NBK, BIP1, BP4, BIK, NBK |
Target/Specificity | This Bik BH3 Domain antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 39-74 amino acids from human Bik BH3 Domain. |
Dilution | WB~~1:2000 IHC-P~~1:50~100 |
Format | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
Precautions | Bik BH3 Domain Antibody is for research use only and not for use in diagnostic or therapeutic procedures. |
Name | BIK {ECO:0000303|PubMed:7478623, ECO:0000312|HGNC:HGNC:1051} |
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Function | Accelerates programmed cell death. Association to the apoptosis repressors Bcl-X(L), BHRF1, Bcl-2 or its adenovirus homolog E1B 19k protein suppresses this death-promoting activity. Does not interact with BAX. |
Cellular Location | Endomembrane system; Single-pass membrane protein. Mitochondrion membrane {ECO:0000250|UniProtKB:O70337}; Single-pass membrane protein. Note=Around the nuclear envelope, and in cytoplasmic membranes. |

Provided below are standard protocols that you may find useful for product applications.
Background
The Bik protein is known to interact with cellular and viral survival-promoting proteins, such as BCL2 and the Epstein-Barr virus in order to enhance programmed cell death. Because its activity is suppressed in the presence of survival-promoting proteins, this protein is suggested as a likely target for antiapoptotic proteins. This protein shares a critical BH3 domain with other death-promoting proteins, BAX and BAK.
References
Arena, V., et al., Genes Chromosomes Cancer 38(1):91-96 (2003).
Gillissen, B., et al., EMBO J. 22(14):3580-3590 (2003).
Germain, M., et al., J. Biol. Chem. 277(20):18053-18060 (2002).
Zou, Y., et al., Cancer Res. 62(1):8-12 (2002).
Castells, A., et al., Gastroenterology 117(4):831-837 (1999).

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