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ROBLD3 Antibody (Center)
Affinity Purified Rabbit Polyclonal Antibody (Pab)
- SPECIFICATION
- CITATIONS
- PROTOCOLS
- BACKGROUND
Application 
| IHC-P, WB, E |
|---|---|
| Primary Accession | Q9Y2Q5 |
| Other Accession | Q3T132, NP_054736.1 |
| Reactivity | Human |
| Predicted | Bovine |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | Rabbit IgG |
| Calculated MW | 13508 Da |
| Antigen Region | 41-70 aa |
| Gene ID | 28956 |
|---|---|
| Other Names | Ragulator complex protein LAMTOR2, Endosomal adaptor protein p14, Late endosomal/lysosomal Mp1-interacting protein, Late endosomal/lysosomal adaptor and MAPK and MTOR activator 2, Mitogen-activated protein-binding protein-interacting protein, MAPBP-interacting protein, Roadblock domain-containing protein 3, LAMTOR2, MAPBPIP, ROBLD3 |
| Target/Specificity | This ROBLD3 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 41-70 amino acids from the Central region of human ROBLD3. |
| Dilution | WB~~1:1000 IHC-P~~1:10~50 |
| Format | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
| Precautions | ROBLD3 Antibody (Center) is for research use only and not for use in diagnostic or therapeutic procedures. |
| Name | LAMTOR2 (HGNC:29796) |
|---|---|
| Synonyms | MAPBPIP, ROBLD3 |
| Function | As part of the Ragulator complex it is involved in amino acid sensing and activation of mTORC1, a signaling complex promoting cell growth in response to growth factors, energy levels, and amino acids (PubMed:20381137, PubMed:28935770, PubMed:29107538, PubMed:29123114, PubMed:29158492). Activated by amino acids through a mechanism involving the lysosomal V-ATPase, the Ragulator plays a dual role for the small GTPases Rag (RagA/RRAGA, RagB/RRAGB, RagC/RRAGC and/or RagD/RRAGD): it (1) acts as a guanine nucleotide exchange factor (GEF), activating the small GTPases Rag and (2) mediates recruitment of Rag GTPases to the lysosome membrane (PubMed:22980980, PubMed:28935770, PubMed:29107538, PubMed:29123114, PubMed:29158492, PubMed:30181260). Activated Ragulator and Rag GTPases function as a scaffold recruiting mTORC1 to lysosomes where it is in turn activated (PubMed:22980980, PubMed:29107538, PubMed:29123114, PubMed:29158492). Adapter protein that enhances the efficiency of the MAP kinase cascade facilitating the activation of MAPK2 (By similarity). |
| Cellular Location | Late endosome membrane {ECO:0000250|UniProtKB:Q9JHS3}; Peripheral membrane protein {ECO:0000250|UniProtKB:Q9JHS3}; Cytoplasmic side {ECO:0000250|UniProtKB:Q9JHS3}. Lysosome membrane; Peripheral membrane protein {ECO:0000250|UniProtKB:Q9JHS3}; Cytoplasmic side {ECO:0000250|UniProtKB:Q9JHS3}. Note=Recruited to lysosome and endosome membranes by LAMTOR1. {ECO:0000250|UniProtKB:Q9JHS3} |
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Provided below are standard protocols that you may find useful for product applications.
Background
The product of this gene is highly conserved with a mouse protein associated with the cytoplasmic face of late endosomes and lysosomes. The mouse protein interacts with MAPK scaffold protein 1, a component of the mitogen-activated protein kinase pathway. In humans, a mutation in this gene has been associated with a primary immunodeficiency syndrome, and suggests a role for this protein in endosomal biogenesis. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq].
References
Sancak, Y., et al. Cell 141(2):290-303(2010)
Wunderlich, W., et al. J. Cell Biol. 152(4):765-776(2001)
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