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NRK Antibody (Center)

Affinity Purified Rabbit Polyclonal Antibody (Pab)

     
  • IF - NRK Antibody (Center) AP13448c
    Fluorescent confocal image of Hela cell stained with NRK Antibody (Center)(Cat#AP13448c).Hela cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with NRK primary antibody (1:25, 1 h at 37℃). For secondary antibody, Alexa Fluor® 488 conjugated donkey anti-rabbit antibody (green) was used (1:400, 50 min at 37℃).Cytoplasmic actin was counterstained with Alexa Fluor® 555 (red) conjugated Phalloidin (7units/ml, 1 h at 37℃). Nuclei were counterstained with DAPI (blue) (10 µg/ml, 10 min). NRK immunoreactivity is localized to Cytoplasm and Nucleus significantly.
    detail
  • WB - NRK Antibody (Center) AP13448c
    NRK Antibody (Center) (Cat. #AP13448c) western blot analysis in A549 cell line lysates (35ug/lane).This demonstrates the NRK antibody detected the NRK protein (arrow).
    detail
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
IF, WB, E
Primary Accession Q7Z2Y5
Other Accession NP_940867.2
Reactivity Human
Host Rabbit
Clonality Polyclonal
Isotype Rabbit IgG
Calculated MW 178479 Da
Antigen Region 721-750 aa
Additional Information
Gene ID 203447
Other Names Nik-related protein kinase, NRK
Target/Specificity This NRK antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 721-750 amino acids from the Central region of human NRK.
Dilution IF~~1:10~50
WB~~1:1000
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsNRK Antibody (Center) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name NRK
Function May phosphorylate cofilin-1 and induce actin polymerization through this process, during the late stages of embryogenesis. Involved in the TNF-alpha-induced signaling pathway (By similarity).
Research Areas
Citations (0)
citation

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Background

The mouse ortholog of this gene encodes a protein kinase required for JNK activation. The encoded protein may be involved in the induction of actin polymerization in late embryogenesis.

References

Ross, M.T., et al. Nature 434(7031):325-337(2005)
Nakano, K., et al. Exp. Cell Res. 287(2):219-227(2003)

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$ 365.00
$ 140.00
Cat# AP13448c
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