NFE2 Antibody (C-term)
Affinity Purified Rabbit Polyclonal Antibody (Pab)
- SPECIFICATION
- CITATIONS
- PROTOCOLS
- BACKGROUND
Application
| WB, E |
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Primary Accession | Q16621 |
Other Accession | NP_001129495.1, NP_006154.1 |
Reactivity | Human |
Host | Rabbit |
Clonality | Polyclonal |
Isotype | Rabbit IgG |
Calculated MW | 41473 Da |
Antigen Region | 312-340 aa |
Gene ID | 4778 |
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Other Names | Transcription factor NF-E2 45 kDa subunit, Leucine zipper protein NF-E2, Nuclear factor, erythroid-derived 2 45 kDa subunit, p45 NF-E2, NFE2 |
Target/Specificity | This NFE2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 312-340 amino acids from the C-terminal region of human NFE2. |
Dilution | WB~~1:1000 |
Format | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
Precautions | NFE2 Antibody (C-term) is for research use only and not for use in diagnostic or therapeutic procedures. |
Name | NFE2 |
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Function | Component of the NF-E2 complex essential for regulating erythroid and megakaryocytic maturation and differentiation. Binds to the hypersensitive site 2 (HS2) of the beta-globin control region (LCR). This subunit (NFE2) recognizes the TCAT/C sequence of the AP-1- like core palindrome present in a number of erythroid and megakaryocytic gene promoters. Requires MAFK or other small MAF proteins for binding to the NF-E2 motif. May play a role in all aspects of hemoglobin production from globin and heme synthesis to procurement of iron. |
Cellular Location | Nucleus, PML body. Cytoplasm. Note=The sumoylated form locates to the nuclear bodies PML oncogenic domains (PODs) Translocated to the cytoplasm through interaction with ITCH |
Tissue Location | Expressed in hematopoietic cells and also in colon and testis |
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Background
Component of the NF-E2 complex essential for regulating erythroid and megakaryocytic maturation and differentiation. Binds to the hypersensitive site 2 (HS2) of the beta-globin control region (LCR). This subunit (NFE2) recognizes the TCAT/C sequence of the AP-1-like core palindrome present in a number of erythroid and megakaryocytic gene promoters. Requires MAFK or other small MAF proteins for binding to the NF-E2 motif. May play a role in all aspects of hemoglobin production from globin and heme synthesis to procurement of iron.
References
Wang, W., et al. Blood 116(2):254-266(2010)
Zhou, Z., et al. J. Biol. Chem. 285(21):15894-15905(2010)
Motohashi, H., et al. Blood 115(3):677-686(2010)
Lee, T.L., et al. Proc. Natl. Acad. Sci. U.S.A. 107(1):52-57(2010)
Soranzo, N., et al. Nat. Genet. 41(11):1182-1190(2009)
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