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Mouse Nek8 Antibody (C-term)
Affinity Purified Rabbit Polyclonal Antibody (Pab)
- SPECIFICATION
- CITATIONS
- PROTOCOLS
- BACKGROUND
Application 
| WB, E |
|---|---|
| Primary Accession | Q91ZR4 |
| Other Accession | NP_543125.1 |
| Reactivity | Human, Mouse |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | Rabbit IgG |
| Calculated MW | 75265 Da |
| Antigen Region | 519-547 aa |
| Gene ID | 140859 |
|---|---|
| Other Names | Serine/threonine-protein kinase Nek8, Never in mitosis A-related kinase 8, NimA-related protein kinase 8, Nek8, Jck |
| Target/Specificity | This Mouse Nek8 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 519-547 amino acids from the C-terminal region of mouse Nek8. |
| Dilution | WB~~1:1000 E~~Use at an assay dependent concentration. |
| Format | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
| Precautions | Mouse Nek8 Antibody (C-term) is for research use only and not for use in diagnostic or therapeutic procedures. |
| Name | Nek8 |
|---|---|
| Synonyms | Jck |
| Function | Required for renal tubular integrity. May regulate local cytoskeletal structure in kidney tubule epithelial cells. May regulate ciliary biogenesis through targeting of proteins to the cilia. Plays a role in organogenesis and is involved in the regulation of the Hippo signaling pathway. |
| Cellular Location | Cytoplasm. Cytoplasm, cytoskeleton. Cell projection, cilium. Cytoplasm, cytoskeleton, microtubule organizing center, centrosome {ECO:0000250|UniProtKB:Q86SG6}. Cytoplasm, cytoskeleton, cilium axoneme {ECO:0000250|UniProtKB:Q86SG6}. Note=Predominantly cytoplasmic Localizes to the proximal region of the primary cilium and is not observed in dividing cells. |
| Tissue Location | Kidney, liver, and testis. |
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Provided below are standard protocols that you may find useful for product applications.
Background
This gene encodes a NIMA-related kinase. Members of this serine/threonine protein kinase family are structurally-related to NIMA (never in mitosis, gene A) which controls mitotic signaling in Aspergillus nidulans.
References
Hellman, N.E., et al. Proc. Natl. Acad. Sci. U.S.A. 107(43):18499-18504(2010)
Ahmadie, R., et al. J. Nutr. 140(8):1438-1444(2010)
Natoli, T.A., et al. Nat. Med. 16(7):788-792(2010)
Shiba, D., et al. Cytoskeleton (Hoboken) 67(2):112-119(2010)
Sohara, E., et al. J. Am. Soc. Nephrol. 19(3):469-476(2008)
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