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BAG2 Antibody (C-term)
Affinity Purified Rabbit Polyclonal Antibody (Pab)
- SPECIFICATION
- CITATIONS: 1
- PROTOCOLS
- BACKGROUND
Application 
| WB, IHC-P, E |
|---|---|
| Primary Accession | O95816 |
| Other Accession | Q91YN9, Q3ZBG5, NP_004273.1 |
| Reactivity | Human |
| Predicted | Bovine, Mouse |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | Rabbit IgG |
| Calculated MW | 23772 Da |
| Antigen Region | 148-176 aa |
| Gene ID | 9532 |
|---|---|
| Other Names | BAG family molecular chaperone regulator 2, BAG-2, Bcl-2-associated athanogene 2, BAG2 |
| Target/Specificity | This BAG2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 148-176 amino acids from the C-terminal region of human BAG2. |
| Dilution | WB~~1:1000 IHC-P~~1:10~50 E~~Use at an assay dependent concentration. |
| Format | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
| Precautions | BAG2 Antibody (C-term) is for research use only and not for use in diagnostic or therapeutic procedures. |
| Name | BAG2 |
|---|---|
| Function | Co-chaperone for HSP70 and HSC70 chaperone proteins. Acts as a nucleotide-exchange factor (NEF) promoting the release of ADP from the HSP70 and HSC70 proteins thereby triggering client/substrate protein release (PubMed:24318877, PubMed:9873016). |
Provided below are standard protocols that you may find useful for product applications.
Background
BAG proteins compete with Hip for binding to the Hsc70/Hsp70 ATPase domain and promote substrate release. All the BAG proteins have an approximately 45-amino acid BAG domain near the C terminus but differ markedly in their N-terminal regions. The predicted BAG2 protein contains 211 amino acids. The BAG domains of BAG1, BAG2, and BAG3 interact specifically with the Hsc70 ATPase domain in vitro and in mammalian cells. All 3 proteins bind with high affinity to the ATPase domain of Hsc70 and inhibit its chaperone activity in a Hip-repressible manner. [provided by RefSeq].
References
Arndt, V., et al. Mol. Biol. Cell 16(12):5891-5900(2005)
Dai, Q., et al. J. Biol. Chem. 280(46):38673-38681(2005)
Ueda, K., et al. J. Biol. Chem. 279(40):41815-41821(2004)
Ueda, K., et al. J. Biol. Chem. 279(40):41815-41821(2004)
Simpson, J.C., et al. EMBO Rep. 1(3):287-292(2000)
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