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ARIH2 Antibody (C-term)
Affinity Purified Rabbit Polyclonal Antibody (Pab)
- SPECIFICATION
- CITATIONS
- PROTOCOLS
- BACKGROUND
Application 
| IHC-P, WB, E |
|---|---|
| Primary Accession | O95376 |
| Other Accession | Q9Z1K6, NP_006312.1 |
| Reactivity | Human |
| Predicted | Mouse |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | Rabbit IgG |
| Calculated MW | 57819 Da |
| Antigen Region | 445-474 aa |
| Gene ID | 10425 |
|---|---|
| Other Names | E3 ubiquitin-protein ligase ARIH2, ARI-2, Protein ariadne-2 homolog, 632-, Triad1 protein, ARIH2, ARI2, TRIAD1 |
| Target/Specificity | This ARIH2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 445-474 amino acids from the C-terminal region of human ARIH2. |
| Dilution | WB~~1:1000 IHC-P~~1:10~50 |
| Format | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
| Precautions | ARIH2 Antibody (C-term) is for research use only and not for use in diagnostic or therapeutic procedures. |
| Name | ARIH2 |
|---|---|
| Synonyms | ARI2, TRIAD1 {ECO:0000303|PubMed:1611831 |
| Function | E3 ubiquitin-protein ligase, which catalyzes ubiquitination of target proteins together with ubiquitin-conjugating enzyme E2 UBE2L3 (PubMed:16118314, PubMed:17646546, PubMed:19340006, PubMed:24076655). Acts as an atypical E3 ubiquitin-protein ligase by working together with cullin-5-RING ubiquitin ligase complex (ECS complex, also named CRL5 complex) and initiating ubiquitination of ECS substrates: associates with ECS complex and specifically mediates addition of the first ubiquitin on ECS targets (By similarity). The initial ubiquitin is then elongated (By similarity). E3 ubiquitin-protein ligase activity is activated upon binding to neddylated form of the ECS complex (PubMed:24076655). Mediates 'Lys-6', 'Lys-48'- and 'Lys-63'-linked polyubiquitination (PubMed:16118314, PubMed:17646546, PubMed:19340006). May play a role in myelopoiesis (PubMed:19340006). |
| Cellular Location | Nucleus. Cytoplasm |
| Tissue Location | Widely expressed with higher expression in granulocytes. |
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Provided below are standard protocols that you may find useful for product applications.
Background
E3 ubiquitin-protein ligase mediating 'Lys-48'-and 'Lys-63'-linked polyubiquitination and subsequent proteasomal degradation of modified proteins. May play a role in myelopoiesis.
References
Marteijn, J.A., et al. Leukemia 23(8):1480-1489(2009)
Marteijn, J.A., et al. Blood 110(9):3128-3135(2007)
Matsuoka, S., et al. Science 316(5828):1160-1166(2007)
Marteijn, J.A., et al. Blood 106(13):4114-4123(2005)
Stelzl, U., et al. Cell 122(6):957-968(2005)
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