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CELA3A Antibody (Center)

Affinity Purified Rabbit Polyclonal Antibody (Pab)

     
  • WB - CELA3A Antibody (Center) AP14493C
    CELA3A Antibody (Center) (Cat. #AP14493c) western blot analysis in Jurkat cell line lysates (35ug/lane).This demonstrates the CELA3A antibody detected the CELA3A protein (arrow).
    detail
  • IHC-P - CELA3A Antibody (Center) AP14493C
    CELA3A Antibody (Center) (Cat. #AP14493c)immunohistochemistry analysis in formalin fixed and paraffin embedded human pancreas tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of CELA3A Antibody (Center) for immunohistochemistry. Clinical relevance has not been evaluated.
    detail
  • SPECIFICATION
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
IHC-P, WB, E
Primary Accession P09093
Other Accession NP_005738.4
Reactivity Human
Host Rabbit
Clonality Polyclonal
Isotype Rabbit IgG
Calculated MW 29489 Da
Antigen Region 157-186 aa
Additional Information
Gene ID 10136
Other Names Chymotrypsin-like elastase family member 3A, Elastase IIIA, Elastase-3A, Protease E, CELA3A, ELA3, ELA3A
Target/Specificity This CELA3A antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 157-186 amino acids from the Central region of human CELA3A.
Dilution WB~~1:1000
IHC-P~~1:10~50
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsCELA3A Antibody (Center) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name CELA3A
Synonyms ELA3, ELA3A
Function Efficient protease with alanine specificity but only little elastolytic activity.
Research Areas
Citations (0)
citation

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Background

Elastases form a subfamily of serine proteases that hydrolyze many proteins in addition to elastin. Humans have six elastase genes which encode the structurally similar proteins elastase 1, 2, 2A, 2B, 3A, and 3B. Unlike other elastases, elastase 3A has little elastolytic activity. Like most of the human elastases, elastase 3A is secreted from the pancreas as a zymogen and, like other serine proteases such as trypsin, chymotrypsin and kallikrein, it has a digestive function in the intestine. Elastase 3A preferentially cleaves proteins after alanine residues. Elastase 3A may also function in the intestinal transport and metabolism of cholesterol. Both elastase 3A and elastase 3B have been referred to as protease E and as elastase 1.

References

Shimada, S., et al. Int. J. Mol. Med. 10(5):599-603(2002)
Shirasu, Y., et al. J. Biochem. 104(2):259-264(1988)
Tani, T., et al. J. Biol. Chem. 263(3):1231-1239(1988)

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$ 365.00
$ 140.00
Cat# AP14493C
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