Foundational characteristics of cancer include proliferation, angiogenesis, migration, evasion of apoptosis, and cellular immortality. Find key markers for these cellular processes and antibodies to detect them.
The SUMOplot™ Analysis Program predicts and scores sumoylation sites in your protein. SUMOylation is a post-translational modification involved in various cellular processes, such as nuclear-cytosolic transport, transcriptional regulation, apoptosis, protein stability, response to stress, and progression through the cell cycle.
The Autophagy Receptor Motif Plotter predicts and scores autophagy receptor binding sites in your protein. Identifying proteins connected to this pathway is critical to understanding the role of autophagy in physiological as well as pathological processes such as development, differentiation, neurodegenerative diseases, stress, infection, and cancer.
Mouse Sgk223 Antibody (N-term)
Affinity Purified Rabbit Polyclonal Antibody (Pab)
- SPECIFICATION
- CITATIONS
- PROTOCOLS
- BACKGROUND
Application 
| WB, E |
|---|---|
| Primary Accession | Q571I4 |
| Other Accession | NP_766499.2 |
| Reactivity | Mouse |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | Rabbit IgG |
| Calculated MW | 147945 Da |
| Antigen Region | 195-224 aa |
| Gene ID | 244418 |
|---|---|
| Other Names | Tyrosine-protein kinase SgK223, Sugen kinase 223, Sgk223, D8Ertd82e |
| Target/Specificity | This Mouse Sgk223 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 195-224 amino acids from the N-terminal region of mouse Sgk223. |
| Dilution | WB~~1:1000 E~~Use at an assay dependent concentration. |
| Format | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
| Precautions | Mouse Sgk223 Antibody (N-term) is for research use only and not for use in diagnostic or therapeutic procedures. |
| Name | Prag1 {ECO:0000312|MGI:MGI:1196223} |
|---|---|
| Function | Catalytically inactive protein kinase that acts as a scaffold protein (By similarity). Functions as an effector of the small GTPase RND2, which stimulates RhoA activity and inhibits NGF-induced neurite outgrowth (By similarity). Promotes Src family kinase (SFK) signaling by regulating the subcellular localization of CSK, a negative regulator of these kinases, leading to the regulation of cell morphology and motility by a CSK-dependent mechanism (By similarity). Acts as a critical coactivator of Notch signaling (PubMed:25038227). |
| Cellular Location | Cytoplasm {ECO:0000250|UniProtKB:D3ZMK9}. Nucleus. Cell junction, focal adhesion {ECO:0000250|UniProtKB:Q86YV5}. Note=Colocalized with NOTCH1 in the nucleus (PubMed:25038227) |
Thousands of laboratories across the world have published research that depended on the performance of antibodies from Abcepta to advance their research. Check out links to articles that cite our products in major peer-reviewed journals, organized by research category.
info@abcepta.com, and receive a free "I Love Antibodies" mug.
Provided below are standard protocols that you may find useful for product applications.
Background
PRAGMIN is also known as SGK223 (sugen kinase 223). The rat homolog was originally identified in a yeast two-hybrid screen for Rnd2 effector proteins expressed in neural cells. Rat Pragmin was found to stimulate RhoA activity for the regulation of neurite outgrowth.
References
Tanaka, H., et al. J. Biol. Chem. 281(15):10355-10364(2006)
If you have used an Abcepta product and would like to share how it has performed, please click on the "Submit Review" button and provide the requested information. Our staff will examine and post your review and contact you if needed.
If you have any additional inquiries please email technical services at tech@abcepta.com.
Ordering Information
Other Products
Shipping Information
