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RANBP3 Antibody (N-term)
Affinity Purified Rabbit Polyclonal Antibody (Pab)
- SPECIFICATION
- CITATIONS
- PROTOCOLS
- BACKGROUND
Application 
| WB, E |
|---|---|
| Primary Accession | Q9H6Z4 |
| Other Accession | NP_015559.2, NP_003615.2, NP_015561.1 |
| Reactivity | Human |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | Rabbit IgG |
| Calculated MW | 60210 Da |
| Antigen Region | 26-55 aa |
| Gene ID | 8498 |
|---|---|
| Other Names | Ran-binding protein 3, RanBP3, RANBP3 |
| Target/Specificity | This RANBP3 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 26-55 amino acids from the N-terminal region of human RANBP3. |
| Dilution | WB~~1:1000 E~~Use at an assay dependent concentration. |
| Format | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
| Precautions | RANBP3 Antibody (N-term) is for research use only and not for use in diagnostic or therapeutic procedures. |
| Name | RANBP3 |
|---|---|
| Function | Acts as a cofactor for XPO1/CRM1-mediated nuclear export, perhaps as export complex scaffolding protein. Bound to XPO1/CRM1, stabilizes the XPO1/CRM1-cargo interaction. In the absence of Ran-bound GTP prevents binding of XPO1/CRM1 to the nuclear pore complex. Binds to CHC1/RCC1 and increases the guanine nucleotide exchange activity of CHC1/RCC1. Recruits XPO1/CRM1 to CHC1/RCC1 in a Ran-dependent manner. Negative regulator of TGF-beta signaling through interaction with the R-SMAD proteins, SMAD2 and SMAD3, and mediating their nuclear export. |
| Cellular Location | Cytoplasm. Nucleus. Note=Nuclear import is promoted by phosphorylation at Ser-126 and is dependent on KPNA4. |
| Tissue Location | Widely expressed with high levels in testis and heart. |
Thousands of laboratories across the world have published research that depended on the performance of antibodies from Abcepta to advance their research. Check out links to articles that cite our products in major peer-reviewed journals, organized by research category.
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Provided below are standard protocols that you may find useful for product applications.
Background
RANBP3 is a protein with a RanBD1 domain that is found in both the nucleus and cytoplasm. This protein plays a role in nuclear export as part of a heteromeric complex. Alternate transcriptional splice variants, encoding different isoforms, have been characterized.
References
Dai, F., et al. Dev. Cell 16(3):345-357(2009)
Yoon, S.O., et al. Mol. Cell 29(3):362-375(2008)
Olsen, J.V., et al. Cell 127(3):635-648(2006)
Hakata, Y., et al. Mol. Cell. Biol. 23(23):8751-8761(2003)
Nemergut, M.E., et al. J. Biol. Chem. 277(20):17385-17388(2002)
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