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ZAR1 Antibody (Center)
Affinity Purified Rabbit Polyclonal Antibody (Pab)
- SPECIFICATION
- CITATIONS
- PROTOCOLS
- BACKGROUND
Application 
| WB, E |
|---|---|
| Primary Accession | Q86SH2 |
| Other Accession | NP_783318.1 |
| Reactivity | Human |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | Rabbit IgG |
| Calculated MW | 45873 Da |
| Antigen Region | 108-137 aa |
| Gene ID | 326340 |
|---|---|
| Other Names | Zygote arrest protein 1, Oocyte-specific maternal effect factor, ZAR1 |
| Target/Specificity | This ZAR1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 108-137 amino acids from the Central region of human ZAR1. |
| Dilution | WB~~1:1000 |
| Format | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
| Precautions | ZAR1 Antibody (Center) is for research use only and not for use in diagnostic or therapeutic procedures. |
| Name | ZAR1 {ECO:0000303|PubMed:12539046, ECO:0000312|HGNC:HGNC:20436} |
|---|---|
| Function | mRNA-binding protein that mediates formation of MARDO (mitochondria-associated ribonucleoprotein domain), a membraneless compartment that stores maternal mRNAs in oocytes. MARDO assembly around mitochondria is directed by an increase in mitochondrial membrane potential during oocyte growth. Promotes formation of MARDO phase-separated membraneless compartment by undergoing liquid-liquid phase separation upon binding to maternal mRNAs. Binds to the 3'-UTR of maternal mRNAs. Maternal mRNAs stored in the MARDO are translationally repressed. Essential for female fertility and oocyte-to-embryo transition by coordinating maternal mRNA storage, translation and degradation. |
| Cellular Location | Cytoplasm, Cytoplasmic ribonucleoprotein granule {ECO:0000250|UniProtKB:Q80SU3}. Cytoplasm {ECO:0000250|UniProtKB:Q80SU3}. Note=Specifically localizes to MARDO (mitochondria-associated ribonucleoprotein domain), a mitochondria- associated membraneless compartment that stores mRNAs in oocytes {ECO:0000250|UniProtKB:Q80SU3} |
| Tissue Location | Ovary and testis.. |
Thousands of laboratories across the world have published research that depended on the performance of antibodies from Abcepta to advance their research. Check out links to articles that cite our products in major peer-reviewed journals, organized by research category.
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Provided below are standard protocols that you may find useful for product applications.
Background
The female gamete, the oocyte, serves the distinct purpose of transmitting the maternal genome and other maternal factors critical for postovulation events. Oocytes have diverse functions in ovarian folliculogenesis, fertilization, and embryogenesis. ZAR1 is an oocyte-specific gene that appears to function at the oocyte-to-gamete transition (Wu et al., 2003 [PubMed 12539046]).
References
Shinojima, Y., et al. J. Dermatol. Sci. 59(2):98-106(2010)
Uzbekova, S., et al. Reprod. Biol. Endocrinol. 4, 12 (2006) :
Wu, X., et al. Biol. Reprod. 69(3):861-867(2003)
Wu, X., et al. Nat. Genet. 33(2):187-191(2003)
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