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BFAR Antibody (N-term)
Affinity Purified Rabbit Polyclonal Antibody (Pab)
- SPECIFICATION
- CITATIONS
- PROTOCOLS
- BACKGROUND
Application 
| WB, E |
|---|---|
| Primary Accession | Q9NZS9 |
| Other Accession | NP_057645.1 |
| Reactivity | Human |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | Rabbit IgG |
| Calculated MW | 52738 Da |
| Antigen Region | 1-30 aa |
| Gene ID | 51283 |
|---|---|
| Other Names | Bifunctional apoptosis regulator, RING finger protein 47, BFAR, BAR, RNF47 |
| Target/Specificity | This BFAR antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 1-30 amino acids from the N-terminal region of human BFAR. |
| Dilution | WB~~1:1000 E~~Use at an assay dependent concentration. |
| Format | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
| Precautions | BFAR Antibody (N-term) is for research use only and not for use in diagnostic or therapeutic procedures. |
| Name | BFAR |
|---|---|
| Synonyms | BAR, RNF47 |
| Function | Membrane-bound E3 ubiquitin ligase that plays a role in several processes including apoptosis regulation or reticulum endoplasmic stress (PubMed:14502241, PubMed:21068390). Has anti- apoptotic activity, both for apoptosis triggered via death-receptors and via mitochondrial factors (PubMed:14502241). Contributes to the dynamic control of IRE1/ERN1 signaling during ER stress by inducing BAX inhibitor 1/TMBIM6 proteasomal degradation (PubMed:21068390). Promotes the activation of TGF-beta signaling by mediating the 'Lys-63'-linked ubiquitination of TGFBR1 which is critical to activate the pathway (PubMed:33914044). Together with NGFR, negatively regulates NF-kappa-B and JNK-related signaling pathways (PubMed:22566094). Promotes the proteasome-mediated degradation of PNPLA3, a protein involveld in lipid metabolism (PubMed:38294943). |
| Cellular Location | Endoplasmic reticulum membrane; Multi-pass membrane protein |
| Tissue Location | Expressed highly in brain, moderately in small intestine, weakly in testes and only faintly in liver and skeletal muscle. Not expressed in heart, kidney, lung and spleen |
Thousands of laboratories across the world have published research that depended on the performance of antibodies from Abcepta to advance their research. Check out links to articles that cite our products in major peer-reviewed journals, organized by research category.
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Provided below are standard protocols that you may find useful for product applications.
Background
BFAR is a apoptosis regulator. Has anti-apoptotic activity, both for apoptosis triggered via death-receptors and via mitochondrial factors.
References
Liu, X., et al. Retina (Philadelphia, Pa.) (2010) In press :
Chua, C.C., et al. Cardiovasc. Res. 81(1):20-27(2009)
Roth, W., et al. Cell Death Differ. 10(10):1178-1187(2003)
Zhang, H., et al. Proc. Natl. Acad. Sci. U.S.A. 97(6):2597-2602(2000)
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