PLEKHF2 Antibody (Center)
Affinity Purified Rabbit Polyclonal Antibody (Pab)
- SPECIFICATION
- CITATIONS
- PROTOCOLS
- BACKGROUND
Application
| WB, E |
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Primary Accession | Q9H8W4 |
Other Accession | NP_078889.1 |
Reactivity | Human |
Host | Rabbit |
Clonality | Polyclonal |
Isotype | Rabbit IgG |
Calculated MW | 27798 Da |
Antigen Region | 71-98 aa |
Gene ID | 79666 |
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Other Names | Pleckstrin homology domain-containing family F member 2, PH domain-containing family F member 2, Endoplasmic reticulum-associated apoptosis-involved protein containing PH and FYVE domains, EAPF, PH and FYVE domain-containing protein 2, Phafin-2, Phafin2, Zinc finger FYVE domain-containing protein 18, PLEKHF2, ZFYVE18 |
Target/Specificity | This PLEKHF2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 71-98 amino acids from the Central region of human PLEKHF2. |
Dilution | WB~~1:1000 |
Format | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
Precautions | PLEKHF2 Antibody (Center) is for research use only and not for use in diagnostic or therapeutic procedures. |
Name | PLEKHF2 |
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Synonyms | ZFYVE18 |
Function | May play a role in early endosome fusion upstream of RAB5, hence regulating receptor trafficking and fluid-phase transport. Enhances cellular sensitivity to TNF-induced apoptosis (PubMed:18288467). |
Cellular Location | Early endosome membrane; Peripheral membrane protein. Endoplasmic reticulum. Note=Colocalizes with EEA1 and RAB5 at endosomal membrane fusion hot spots (PubMed:19995552). May translocate to the endoplasmic reticulum in the early phase of apoptosis (PubMed:18288467). |
Tissue Location | Expressed in placenta, ovary and small intestine, as well as in heart and pancreas. Also expressed in peripheral blood mononuclear cells and dendritic cells. |
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Provided below are standard protocols that you may find useful for product applications.
Background
PLEKHF2 (Phafin 2) is a PH and FYVE domain containing protein 2. The FYVE domain and Zinc binding domain targets proteins to membrane lipids via interaction with phosphatidylinositol 3 phosphate, PI3P. The FYVE domain and Zinc binding domain present in Fab1, YOTB, Vac1, and EEA1.
References
Lin, W.J., et al. Biochem. Biophys. Res. Commun. 391(1):1043-1048(2010)
Li, C., et al. J. Mol. Med. 86(4):471-484(2008)
Olsen, J.V., et al. Cell 127(3):635-648(2006)
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