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PPIE Antibody (N-term)

Affinity Purified Rabbit Polyclonal Antibody (Pab)

     
  • WB - PPIE Antibody (N-term) AP17550a
    PPIE Antibody (N-term) (Cat. #AP17550a) western blot analysis in U937 cell line lysates (35ug/lane).This demonstrates the PPIE antibody detected the PPIE protein (arrow).
    detail
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
WB, E
Primary Accession Q9UNP9
Other Accession Q9QZH3, A4FV72, NP_982281.1, NP_006103.1
Reactivity Human
Predicted Bovine, Mouse
Host Rabbit
Clonality Polyclonal
Isotype Rabbit IgG
Calculated MW 33431 Da
Antigen Region 22-49 aa
Additional Information
Gene ID 10450
Other Names Peptidyl-prolyl cis-trans isomerase E, PPIase E, Cyclophilin E, Cyclophilin-33, Rotamase E, PPIE, CYP33
Target/Specificity This PPIE antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 22-49 amino acids from the N-terminal region of human PPIE.
Dilution WB~~1:1000
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsPPIE Antibody (N-term) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name PPIE
Synonyms CYP33 {ECO:0000303|PubMed:8977107}
Function Involved in pre-mRNA splicing as component of the spliceosome (PubMed:11991638, PubMed:28076346). Combines RNA-binding and PPIase activities (PubMed:8977107, PubMed:18258190, PubMed:20677832, PubMed:20460131). Binds mRNA and has a preference for single-stranded RNA molecules with poly-A and poly-U stretches, suggesting it binds to the poly(A)-region in the 3'-UTR of mRNA molecules (PubMed:8977107, PubMed:18258190, PubMed:20460131). Catalyzes the cis-trans isomerization of proline imidic peptide bonds in proteins (PubMed:8977107, PubMed:18258190, PubMed:20677832, PubMed:20541251). Inhibits KMT2A activity; this requires proline isomerase activity (PubMed:20677832, PubMed:20541251, PubMed:20460131).
Cellular Location Nucleus
Tissue Location Found in all the examined tissues including heart, brain, placenta, lung, liver, skeletal muscle, kidney and pancreas
Research Areas
Citations (0)
citation

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Background

The protein encoded by this gene is a member of the peptidyl-prolyl cis-trans isomerase (PPIase) family. PPIases catalyze the cis-trans isomerization of proline imidic peptide bonds in oligopeptides and accelerate the folding of proteins. This protein contains a highly conserved cyclophilin (CYP) domain as well as an RNA-binding domain. It was shown to possess PPIase and protein folding activities, and it also exhibits RNA-binding activity. Alternative splicing results in multiple transcript variants. A related pseudogene, which is also located on chromosome 1, has been identified.

References

Park, S., et al. Biochemistry 49(31):6576-6586(2010)
Hom, R.A., et al. J. Mol. Biol. 400(2):145-154(2010)
Wang, Z., et al. Cell 141(7):1183-1194(2010)
Naukkarinen, J., et al. PLoS Genet. 6 (6), E1000976 (2010) :
Chen, J., et al. Cancer Res. 68(15):6199-6207(2008)

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$ 182.50
$ 70.00
Cat# AP17550a
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