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B4GALNT1 Antibody (Center)

Affinity Purified Rabbit Polyclonal Antibody (Pab)

     
  • WB - B4GALNT1 Antibody (Center) AP18357C
    B4GALNT1 Antibody (Center) (Cat. #AP18357c) western blot analysis in mouse Neuro-2a cell line lysates (35ug/lane).This demonstrates the B4GALNT1 Antibody detected the B4GALNT1 protein (arrow).
    detail
  • WB - B4GALNT1 Antibody (Center) AP18357C
    B4GALNT1 Antibody (Center) (Cat. #AP18357c) western blot analysis in MDA-MB453 cell line lysates (35ug/lane).This demonstrates the B4GALNT1 antibody detected the B4GALNT1 protein (arrow).
    detail
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
WB, E
Primary Accession Q00973
Other Accession NP_001469.1
Reactivity Human, Mouse
Host Rabbit
Clonality Polyclonal
Isotype Rabbit IgG
Calculated MW 58882 Da
Antigen Region 296-323 aa
Additional Information
Gene ID 2583
Other Names Beta-1, 4 N-acetylgalactosaminyltransferase 1, (N-acetylneuraminyl)-galactosylglucosylceramide, GM2/GD2 synthase, GalNAc-T, B4GALNT1, GALGT, SIAT2
Target/Specificity This B4GALNT1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 296-323 amino acids from the Central region of human B4GALNT1.
Dilution WB~~1:1000
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsB4GALNT1 Antibody (Center) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name B4GALNT1 (HGNC:4117)
Synonyms GALGT, SIAT2
Function Involved in the biosynthesis of gangliosides GM2, GD2, GT2 and GA2 from GM3, GD3, GT3 and GA3, respectively.
Cellular Location Golgi apparatus membrane {ECO:0000250|UniProtKB:Q10468}; Single-pass type II membrane protein
Research Areas
Citations (0)
citation

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Background

GM2 and GD2 gangliosides are sialic acid-containing glycosphingolipids. GalNAc-T is the enzyme involved in the biosynthesis of G(M2) and G(D2) glycosphingolipids. GalNAc-T catalyzes the transfer of GalNAc into G(M3) and G(D3) by a beta-1,4 linkage, resulting in the synthesis of G(M2) and G(D2), respectively.

References

Chen, L.C., et al. Lung Cancer 67(2):216-220(2010)
Boraska, V., et al. Clin. Biochem. 42(9):819-822(2009)
Gornati, R., et al. Mol. Cell. Biochem. 298 (1-2), 59-68 (2007) :
Li, J., et al. J. Biol. Chem. 275(52):41476-41486(2000)
Li, J., et al. J. Biol. Chem. 275(52):41476-41486(2000)

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$ 365.00
$ 140.00
Cat# AP18357C
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