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ECH1 Antibody (C-term)

Affinity Purified Rabbit Polyclonal Antibody (Pab)

     
  • WB - ECH1 Antibody (C-term) AP18579b
    ECH1 Antibody (C-term) (Cat. #AP18579b) western blot analysis in A549 cell line lysates (35ug/lane).This demonstrates the ECH1 antibody detected the ECH1 protein (arrow).
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
WB, E
Primary Accession Q13011
Other Accession NP_001389.2
Reactivity Human
Host Rabbit
Clonality Polyclonal
Isotype Rabbit IgG
Calculated MW 35816 Da
Antigen Region 223-250 aa
Additional Information
Gene ID 1891
Other Names Delta(3, 5)-Delta(2, 4)-dienoyl-CoA isomerase, mitochondrial, 533-, ECH1
Target/Specificity This ECH1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 223-250 amino acids from the C-terminal region of human ECH1.
Dilution WB~~1:1000
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsECH1 Antibody (C-term) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name ECH1 (HGNC:3149)
Function Isomerization of 3-trans,5-cis-dienoyl-CoA to 2-trans,4- trans-dienoyl-CoA.
Cellular Location Mitochondrion {ECO:0000250|UniProtKB:Q62651}. Peroxisome {ECO:0000250|UniProtKB:Q62651}
Research Areas
Citations (0)
citation

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Background

This gene encodes a member of the hydratase/isomerase superfamily. The gene product shows high sequence similarity to enoyl-coenzyme A (CoA) hydratases of several species, particularly within a conserved domain characteristic of these proteins. The encoded protein, which contains a C-terminal peroxisomal targeting sequence, localizes to the peroxisome. The rat ortholog, which localizes to the matrix of both the peroxisome and mitochondria, can isomerize 3-trans,5-cis-dienoyl-CoA to 2-trans,4-trans-dienoyl-CoA, indicating that it is a delta3,5-delta2,4-dienoyl-CoA isomerase. This enzyme functions in the auxiliary step of the fatty acid beta-oxidation pathway. Expression of the rat gene is induced by peroxisome proliferators.

References

Olsen, J.V., et al. Cell 127(3):635-648(2006)
Olsen, J.V., et al. Cell 127(3):635-648(2006)
Kovalyov, L.I., et al. Biochemistry Mosc. 71(4):448-453(2006)
Goehler, H., et al. Mol. Cell 15(6):853-865(2004)
Jia, Y., et al. J. Biol. Chem. 278(47):47232-47239(2003)

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$ 182.50
$ 70.00
Cat# AP18579b
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