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Mouse Mcm2 Antibody (C-term)
Affinity Purified Rabbit Polyclonal Antibody (Pab)
- SPECIFICATION
- CITATIONS
- PROTOCOLS
- BACKGROUND
Application 
| WB, E |
|---|---|
| Primary Accession | P97310 |
| Other Accession | NP_032590.2 |
| Reactivity | Mouse |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | Rabbit IgG |
| Calculated MW | 102078 Da |
| Antigen Region | 713-742 aa |
| Gene ID | 17216 |
|---|---|
| Other Names | DNA replication licensing factor MCM2, Minichromosome maintenance protein 2 homolog, Nuclear protein BM28, Mcm2, Bm28, Cdcl1, Kiaa0030, Mcmd2 |
| Target/Specificity | This Mouse Mcm2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 713-742 amino acids from the C-terminal region of mouse Mcm2. |
| Dilution | WB~~1:1000 |
| Format | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
| Precautions | Mouse Mcm2 Antibody (C-term) is for research use only and not for use in diagnostic or therapeutic procedures. |
| Name | Mcm2 |
|---|---|
| Synonyms | Bm28, Cdcl1, Kiaa0030, Mcmd2 |
| Function | Acts as a component of the MCM2-7 complex (MCM complex) which is the replicative helicase essential for 'once per cell cycle' DNA replication initiation and elongation in eukaryotic cells. Core component of CDC45-MCM-GINS (CMG) helicase, the molecular machine that unwinds template DNA during replication, and around which the replisome is built. The active ATPase sites in the MCM2-7 ring are formed through the interaction surfaces of two neighboring subunits such that a critical structure of a conserved arginine finger motif is provided in trans relative to the ATP-binding site of the Walker A box of the adjacent subunit. The six ATPase active sites, however, are likely to contribute differentially to the complex helicase activity. Required for the entry in S phase and for cell division (PubMed:10567526). Plays a role in terminally differentiated hair cells development of the cochlea and induces cells apoptosis (By similarity). |
| Cellular Location | Nucleus {ECO:0000250|UniProtKB:P49736}. Chromosome {ECO:0000250|UniProtKB:P49736}. Note=Associated with chromatin before the formation of nuclei and detaches from it as DNA replication progresses. {ECO:0000250|UniProtKB:P55861} |
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Provided below are standard protocols that you may find useful for product applications.
Background
Mcm2 acts as a factor that allows the DNA to undergo a single round of replication per cell cycle. Required for the entry in S phase and for cell division.
References
Kunnev, D., et al. Oncogene 29(25):3630-3638(2010)
Chuang, C.H., et al. PLoS Genet. 6 (9) (2010) :
Lee, E.Y., et al. Dev. Biol. 332(1):104-115(2009)
Hasegawa, M., et al. Leuk. Res. 33(8):1100-1107(2009)
Breunig, J.J., et al. Proc. Natl. Acad. Sci. U.S.A. 105(35):13127-13132(2008)
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