Mouse Cbx5 Antibody (C-term)
Affinity Purified Rabbit Polyclonal Antibody (Pab)
- SPECIFICATION
- CITATIONS
- PROTOCOLS
- BACKGROUND
Application
| WB, E |
---|---|
Primary Accession | Q61686 |
Other Accession | P45973, NP_001070257.1 |
Reactivity | Mouse |
Predicted | Human |
Host | Rabbit |
Clonality | Polyclonal |
Isotype | Rabbit IgG |
Calculated MW | 22186 Da |
Antigen Region | 160-186 aa |
Gene ID | 12419 |
---|---|
Other Names | Chromobox protein homolog 5, Heterochromatin protein 1 homolog alpha, HP1 alpha, Cbx5, Hp1a |
Target/Specificity | This Mouse Cbx5 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 160-186 amino acids from the C-terminal region of mouse Cbx5. |
Dilution | WB~~1:1000 |
Format | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
Precautions | Mouse Cbx5 Antibody (C-term) is for research use only and not for use in diagnostic or therapeutic procedures. |
Name | Cbx5 |
---|---|
Synonyms | Hp1a |
Function | Component of heterochromatin that recognizes and binds histone H3 tails methylated at 'Lys-9' (H3K9me), leading to epigenetic repression. In contrast, it is excluded from chromatin when 'Tyr-41' of histone H3 is phosphorylated (H3Y41ph). Can interact with lamin-B receptor (LBR). This interaction can contribute to the association of the heterochromatin with the inner nuclear membrane. Involved in the formation of functional kinetochore through interaction with MIS12 complex proteins (By similarity). |
Cellular Location | Nucleus {ECO:0000250|UniProtKB:P45973}. Chromosome {ECO:0000250|UniProtKB:P45973}. Chromosome, centromere {ECO:0000250|UniProtKB:P45973}. Note=Colocalizes with HNRNPU in the nucleus (By similarity). Component of centromeric and pericentromeric heterochromatin. Associates with chromosomes during mitosis. Associates specifically with chromatin during metaphase and anaphase. Localizes to sites of DNA damage. {ECO:0000250|UniProtKB:P45973} |
Thousands of laboratories across the world have published research that depended on the performance of antibodies from Abcepta to advance their research. Check out links to articles that cite our products in major peer-reviewed journals, organized by research category.
info@abcepta.com, and receive a free "I Love Antibodies" mug.
Provided below are standard protocols that you may find useful for product applications.
Background
Component of heterochromatin that recognizes and binds histone H3 tails methylated at 'Lys-9' (H3K9me), leading to epigenetic repression. In contrast, it is excluded from chromatin when 'Tyr-41' of histone H3 is phosphorylated (H3Y41ph). Can interact with lamin-B receptor (LBR). This interaction can contribute to the association of the heterochromatin with the inner nuclear membrane. Involved in the formation of functional kinetochore through interaction with MIS12 complex proteins (By similarity).
References
Sun, Y., et al. Biophys. J. 99(4):1274-1283(2010)
Muller, K.P., et al. Biophys. J. 97(11):2876-2885(2009)
Dong, K.B., et al. EMBO J. 27(20):2691-2701(2008)
Villeneuve, L.M., et al. Proc. Natl. Acad. Sci. U.S.A. 105(26):9047-9052(2008)
Brancorsini, S., et al. Cell Cycle 7(10):1415-1422(2008)
If you have used an Abcepta product and would like to share how it has performed, please click on the "Submit Review" button and provide the requested information. Our staff will examine and post your review and contact you if needed.
If you have any additional inquiries please email technical services at tech@abcepta.com.