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NUP54 Antibody (Center)

Purified Rabbit Polyclonal Antibody (Pab)

     
  • WB - NUP54 Antibody (Center) AP1924b
    Western blot analysis of anti-NUP54 Pab (Cat. #AP1924b) in mouse heart tissue lysate.NUP54(arrow) was detected using the purified Pab.
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  • SPECIFICATION
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
WB, E
Primary Accession Q7Z3B4
Reactivity Human
Host Rabbit
Clonality Polyclonal
Isotype Rabbit IgG
Calculated MW 55435 Da
Antigen Region 211-243 aa
Additional Information
Gene ID 53371
Other Names Nucleoporin p54, 54 kDa nucleoporin, NUP54
Target/Specificity This NUP54 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 211-243 amino acids from the Central region of human NUP54.
Dilution WB~~1:1000
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsNUP54 Antibody (Center) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name NUP54
Function Component of the nuclear pore complex, a complex required for the trafficking across the nuclear membrane.
Cellular Location Nucleus, nuclear pore complex {ECO:0000250|UniProtKB:P70582}. Nucleus membrane {ECO:0000250|UniProtKB:P70582}; Peripheral membrane protein {ECO:0000250|UniProtKB:P70582}; Cytoplasmic side {ECO:0000250|UniProtKB:P70582}. Nucleus membrane {ECO:0000250|UniProtKB:P70582}; Peripheral membrane protein {ECO:0000250|UniProtKB:P70582}; Nucleoplasmic side {ECO:0000250|UniProtKB:P70582}. Note=Biased towards cytoplasmic side Central region of the nuclear pore complex, within the transporter {ECO:0000250|UniProtKB:P70582}
Research Areas
Citations (0)
citation

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Background

The nuclear envelope creates distinct nuclear and cytoplasmic compartments in eukaryotic cells. It consists of two concentric membranes perforated by nuclear pores, large protein complexes that form aqueous channels to regulate the flow of macromolecules between the nucleus and the cytoplasm. These complexes are composed of at least 100 different polypeptide subunits, many of which belong to the nucleoporin family. NUP54 is a member of the phe-gly (FG) repeat-containing nucleoporin subset.

References

Stoffler, D., et al., Curr. Opin. Cell Biol. 11(3):391-401 (1999).
Bodoor, K., et al., Biochem. Cell Biol. 77(4):321-329 (1999).
Popov, S., et al., J. Biol. Chem. 273(21):13347-13352 (1998).
Hu, T., et al., J. Cell Biol. 134(3):589-601 (1996).
Finlay, D.R., et al., J. Cell Biol. 114(1):169-183 (1991).

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$ 182.50
$ 70.00
Cat# AP1924b
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