SMG5 Antibody (C-term)
Affinity Purified Rabbit Polyclonal Antibody (Pab)
- SPECIFICATION
- CITATIONS
- PROTOCOLS
- BACKGROUND
Application
| WB, E |
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Primary Accession | Q9UPR3 |
Other Accession | Q6ZPY2, NP_056142.2 |
Reactivity | Human |
Predicted | Mouse |
Host | Rabbit |
Clonality | Polyclonal |
Isotype | Rabbit IgG |
Calculated MW | 113928 Da |
Antigen Region | 912-941 aa |
Gene ID | 23381 |
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Other Names | Protein SMG5, EST1-like protein B, LPTS-RP1, LPTS-interacting protein, SMG-5 homolog, hSMG-5, SMG5, EST1B, KIAA1089 |
Target/Specificity | This SMG5 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 912-941 amino acids from the C-terminal region of human SMG5. |
Dilution | WB~~1:1000 |
Format | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
Precautions | SMG5 Antibody (C-term) is for research use only and not for use in diagnostic or therapeutic procedures. |
Name | SMG5 (HGNC:24644) |
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Function | Plays a role in nonsense-mediated mRNA decay. Does not have RNase activity by itself. Promotes dephosphorylation of UPF1. Together with SMG7 is thought to provide a link to the mRNA degradation machinery involving exonucleolytic pathways, and to serve as an adapter for UPF1 to protein phosphatase 2A (PP2A), thereby triggering UPF1 dephosphorylation. Necessary for TERT activity. |
Cellular Location | Cytoplasm. Nucleus. Note=Predominantly cytoplasmic, and nuclear. Shuttles between nucleus and cytoplasm. Detected in cytoplasmic mRNA decay bodies |
Tissue Location | Ubiquitous. |
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Provided below are standard protocols that you may find useful for product applications.
Background
SMG5 is involved in nonsense-mediated mRNA decay (Ohnishi et al., 2003 [PubMed 14636577]).
References
Kamatani, Y., et al. Nat. Genet. 42(3):210-215(2010)
Glavan, F., et al. EMBO J. 25(21):5117-5125(2006)
Lee, H., et al. Mol. Cell. Biol. 26(14):5259-5269(2006)
Azzalin, C.M., et al. Curr. Biol. 16(4):433-439(2006)
Unterholzner, L., et al. Mol. Cell 16(4):587-596(2004)
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