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CD73 (NT5E) Antibody (N-term)
Purified Rabbit Polyclonal Antibody (Pab)
- SPECIFICATION
- CITATIONS: 4
- PROTOCOLS
- BACKGROUND
Application 
| IHC-P, E |
|---|---|
| Primary Accession | P21589 |
| Reactivity | Human |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | Rabbit IgG |
| Calculated MW | 63368 Da |
| Antigen Region | 1-30 aa |
| Gene ID | 4907 |
|---|---|
| Other Names | 5'-nucleotidase, 5'-NT, Ecto-5'-nucleotidase, CD73, NT5E, NT5, NTE |
| Target/Specificity | This CD73 (NT5E) antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 1-30 amino acids from the N-terminal region of human CD73 (NT5E). |
| Dilution | IHC-P~~1:10~50 E~~Use at an assay dependent concentration. |
| Format | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS. |
| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
| Precautions | CD73 (NT5E) Antibody (N-term) is for research use only and not for use in diagnostic or therapeutic procedures. |
| Name | NT5E |
|---|---|
| Synonyms | NT5, NTE |
| Function | Catalyzes the hydrolysis of nucleotide monophosphates, releasing inorganic phosphate and the corresponding nucleoside, with AMP being the preferred substrate (PubMed:21933152, PubMed:22997138, PubMed:23142347, PubMed:24887587, PubMed:34403084). Shows a preference for ribonucleotide monophosphates over their equivalent deoxyribose forms (PubMed:34403084). Other substrates include IMP, UMP, GMP, CMP, dAMP, dCMP, dTMP, NAD and NMN (PubMed:21933152, PubMed:22997138, PubMed:23142347, PubMed:24887587, PubMed:34403084). |
| Cellular Location | Cell membrane; Lipid-anchor, GPI-anchor |
Provided below are standard protocols that you may find useful for product applications.
Background
Ecto-5-prime-nucleotidase catalyzes conversion at neutral pH of purine 5-prime mononucleotides to nucleosides. The enzyme consists of a dimer of 2 identical 70 kD subunits tethered by a glycosyl phosphatidyl inositol moeity to the exterior plasma membrane surface. The enzyme, a marker of lymphocyte differentiation is associated in deficiency with a variety of immunodeficiency diseases. The preferred substrate is AMP. The NT5 gene has been localized to 6q14-q21 by immunofluorescence and a study of a panel of human x mouse hybrids that contained fragments of chromosome 6 as translocations.
References
Hashikawa, T., et al., J. Dent. Res. 82(11):888-892 (2003).
Rosi, F., et al., Biomed. Pharmacother. 56(2):100-104 (2002).
Misumi, Y., et al., Eur. J. Biochem. 191(3):563-569 (1990).
Boyle, J.M., et al., Hum. Genet. 81(1):88-92 (1988).
Kalsi, K., et al., Mol. Cell. Biochem. 232 (1-2), 113-119 (2002).
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