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CD73 (NT5E) Antibody (C-term)

Purified Rabbit Polyclonal Antibody (Pab)

  • WB - CD73 (NT5E) Antibody (C-term) AP2014b
    Western blot analysis of NT5E (arrow) using rabbit polyclonal NT5E Antibody (M535) (Cat. #AP2014b). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the NT5E gene.
  • WB - CD73 (NT5E) Antibody (C-term) AP2014b
    Western blot analysis of CD73 Pab (Cat. #AP2014b) in mouse brain tissue lysate. CD73 (Arrow) was detected using purified Pab. Secondary HRP-anti-rabbit was used for signal visualization with chemiluminescence.
  • WB - CD73 (NT5E) Antibody (C-term) AP2014b
    Western blot analysis of lysates from U266B1,HepG2 cell line,mouse brain tissue lysate(from left to right), using NT5E-M535 Antibody(Cat. #AP2014b). AP2014b was diluted at 1:2000 at each lane. A goat anti-rabbit IgG H&L(HRP) at 1:10000 dilution was used as the secondary antibody.Lysates at 20ug per lane.
  • WB - CD73 (NT5E) Antibody (C-term) AP2014b
    All lanes : Anti-NT5E Antibody (M535) at 1:1000 dilution Lane 1: HepG2 whole cell lysate Lane 2: U266B1 whole cell lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 63 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
  • IHC-P - CD73 (NT5E) Antibody (C-term) AP2014b
    Formalin-fixed and paraffin-embedded human Placenta tissue reacted with CD73 (NT5E) Antibody (C-term)(Cat.#AP2014b), which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
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Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession P21589
Other Accession NP_002517
Reactivity Human, Mouse
Host Rabbit
Clonality Polyclonal
Isotype Rabbit IgG
Calculated MW 63368 Da
Antigen Region 520-550 aa
Additional Information
Gene ID 4907
Other Names 5'-nucleotidase, 5'-NT, Ecto-5'-nucleotidase, CD73, NT5E, NT5, NTE
Target/Specificity This CD73 (NT5E) antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 520-550 amino acids from the C-terminal region of human CD73 (NT5E).
Dilution WB~~1:1000
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsCD73 (NT5E) Antibody (C-term) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name NT5E
Synonyms NT5, NTE
Function Catalyzes the hydrolysis of nucleotide monophosphates, releasing inorganic phosphate and the corresponding nucleoside, with AMP being the preferred substrate (PubMed:21933152, PubMed:34403084, PubMed:24887587, PubMed:22997138, PubMed:23142347). Shows a preference for ribonucleotide monophosphates over their equivalent deoxyribose forms (PubMed:34403084). Other substrates include IMP, UMP, GMP, CMP, dAMP, dCMP, dTMP, NAD and NMN (PubMed:21933152, PubMed:34403084, PubMed:24887587, PubMed:22997138, PubMed:23142347).
Cellular Location Cell membrane; Lipid-anchor, GPI-anchor
Research Areas
Citations ( 0 )


Ecto-5-prime-nucleotidase (5-prime-ribonucleotide phosphohydrolase) catalyzes the conversion at neutral pH of purine 5-prime mononucleotides to nucleosides, the preferred substrate being AMP. The enzyme consists of a dimer of 2 identical 70 kD subunits bound externally to the plasma membrane by a glycosyl phosphatidyl inositol linkage. The enzyme is used as a marker of lymphocyte differentiation. Consequently, a deficiency of NT5E occurs in a variety of immunodeficiency diseases. Other forms of 5-prime nucleotidase exist in the cytoplasm and lysosomes and can be distinguished from ecto-NT5 by their substrate affinities, requirement for divalent magnesium ion, activation by ATP, and inhibition by inorganic phosphate. It is not known whether the different enzymes are coded by different genes or result from different posttranslational modifications of a single coding sequence.


Hashikawa, T., et al., J. Dent. Res. 82(11):888-892 (2003). Rosi, F., et al., Biomed. Pharmacother. 56(2):100-104 (2002). Misumi, Y., et al., Eur. J. Biochem. 191(3):563-569 (1990). Boyle, J.M., et al., Hum. Genet. 81(1):88-92 (1988). Kalsi, K., et al., Mol. Cell. Biochem. 232 (1-2), 113-119 (2002).

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$ 365.00
$ 140.00
Cat# AP2014b
Availability: In Stock
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