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SLC28A1 Antibody (Center)

Affinity Purified Rabbit Polyclonal Antibody (Pab)

     
  • WB - SLC28A1 Antibody (Center) AP20317c
    SLC28A1 Antibody (Center) (Cat. #AP20317c) western blot analysis in ZR-75-1 cell line lysates (35ug/lane).This demonstrates the SLC28A1 antibody detected the SLC28A1 protein (arrow).
    detail
  • WB - SLC28A1 Antibody (Center) AP20317c
    All lanes : Anti-SLC28A1 Antibody (Center) at 1:2000 dilution Lane 1: ZR-75-1 whole cell lysate Lane 2: 293T/17 whole cell lysate Lane 3: HT-29 whole cell lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 72 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
    detail
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
WB, E
Primary Accession O00337
Other Accession O62667
Reactivity Human
Predicted Pig
Host Rabbit
Clonality Polyclonal
Isotype Rabbit IgG
Calculated MW 71584 Da
Antigen Region 381-409 aa
Additional Information
Gene ID 9154
Other Names Sodium/nucleoside cotransporter 1, Concentrative nucleoside transporter 1, CNT 1, hCNT1, Na(+)/nucleoside cotransporter 1, Sodium-coupled nucleoside transporter 1, Solute carrier family 28 member 1, SLC28A1, CNT1
Target/Specificity This SLC28A1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 381-409 amino acids from the Central region of human SLC28A1.
Dilution WB~~1:2000
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsSLC28A1 Antibody (Center) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name SLC28A1 (HGNC:11001)
Function Sodium and pyrimidine nucleoside symporter of the plasma membrane that imports uridine, thymidine and cytidine into cells by coupling their transport to the transmembrane sodium electrochemical gradient. Also transports adenosine, an atypical substrate transported with high apparent affinity, but low maximum velocity. Therefore, exhibits the transport characteristics of the nucleoside transport system cit or N2 subtype (N2/cit) (PubMed:9124315, PubMed:10455109, PubMed:14701834, PubMed:15194733, PubMed:21795683, PubMed:21998139, PubMed:32126230, PubMed:30658162). Involved in renal nucleoside (re)absorption (PubMed:30658162).
Cellular Location Cell membrane; Multi-pass membrane protein {ECO:0000250|UniProtKB:Q62674}. Apical cell membrane; Multi-pass membrane protein {ECO:0000250|UniProtKB:Q62674}
Tissue Location Expressed in kidney..
Citations (0)
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Background

Sodium-dependent and pyrimidine-selective. Exhibits the transport characteristics of the nucleoside transport system cit or N2 subtype (N2/cit) (selective for pyrimidine nucleosides and adenosine). It also transports the antiviral pyrimidine nucleoside analogs 3'-azido-3'-deoxythymidine (AZT) and 2',3'-dideoxycytidine (ddC). It may be involved in the intestinal absorption and renal handling of pyrimidine nucleoside analogs used to treat acquired immunodeficiency syndrome (AIDS). It has the following selective inhibition: adenosine, thymidine, cytidine, uridine >> guanosine, inosine.

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$ 365.00
$ 140.00
Cat# AP20317c
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