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POLR2C Antibody (C-term)

Affinity Purified Rabbit Polyclonal Antibody (Pab)

     
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  • IF - POLR2C Antibody (C-term) AP20520b
    Fluorescent image of Hela cells stained with POLR2C Antibody (C-term)(Cat#AP20520B). AP20520B was diluted at 1:25 dilution. An Alexa Fluor 488-conjugated goat anti-rabbit lgG at 1:400 dilution was used as the secondary antibody (green). Cytoplasmic actin was counterstained with Alexa Fluor® 555 conjugated with Phalloidin (red).
    detail
  • WB - POLR2C Antibody (C-term) AP20520b
    POLR2C Antibody (C-term) (Cat. #AP20520b) western blot analysis in Hela,U251 cell line and mouse skeletal muscle tissue lysates (35ug/lane).This demonstrates the POLR2C antibody detected the POLR2C protein (arrow).
    detail
  • IHC-P - POLR2C Antibody (C-term) AP20520b
    Formalin-fixed and paraffin-embedded H.brain, H.cerebellum tissue reacted with POLR2C Antibody (C-term) (Cat#AP20520b).
    detail
  • SPECIFICATION
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
IF, WB, IHC-P, E
Primary Accession P19387
Other Accession P97760, Q3T0Q3
Reactivity Human, Mouse
Predicted Bovine
Host Rabbit
Clonality Polyclonal
Isotype Rabbit IgG
Calculated MW 31441 Da
Antigen Region 200-228 aa
Additional Information
Gene ID 5432
Other Names DNA-directed RNA polymerase II subunit RPB3, RNA polymerase II subunit 3, RNA polymerase II subunit B3, DNA-directed RNA polymerase II 33 kDa polypeptide, RPB33, DNA-directed RNA polymerase II subunit C, RPB31, POLR2C
Target/Specificity This POLR2C antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 200-228 amino acids from the C-terminal region of human POLR2C.
Dilution IF~~1:25
WB~~1:1000
IHC-P~~1:100
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsPOLR2C Antibody (C-term) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name POLR2C (HGNC:9189)
Function DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates. Component of RNA polymerase II which synthesizes mRNA precursors and many functional non-coding RNAs. Pol II is the central component of the basal RNA polymerase II transcription machinery. It is composed of mobile elements that move relative to each other. RPB3 is part of the core element with the central large cleft and the clamp element that moves to open and close the cleft (By similarity).
Cellular Location Nucleus.
Research Areas
Citations (0)
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Background

DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates. Component of RNA polymerase II which synthesizes mRNA precursors and many functional non-coding RNAs. Pol II is the central component of the basal RNA polymerase II transcription machinery. It is composed of mobile elements that move relative to each other. RPB3 is part of the core element with the central large cleft and the clamp element that moves to open and close the cleft (By similarity).

References

Pati U.K., et al. J. Biol. Chem. 265:8400-8403(1990).
Dammann R., et al. Biochim. Biophys. Acta 1396:153-157(1998).
Bruno T., et al. Submitted (FEB-1998) to the EMBL/GenBank/DDBJ databases.
Loftus B.J., et al. Genomics 60:295-308(1999).
Bienvenut W.V., et al. Submitted (MAR-2009) to UniProtKB.

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$ 365.00
$ 140.00
Cat# AP20520b
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