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Mouse T Antibody (C-term)

Purified Rabbit Polyclonal Antibody (Pab)

     
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  • WB - Mouse T Antibody (C-term) AP20762c
    Western blot analysis of lysates from mouse pancrease and mouse liver tissue lysate (from left to right), using Mouse T Antibody (C-term)(Cat. #AP20762c). AP20762c was diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L(HRP) at 1:5000 dilution was used as the secondary antibody. Lysates at 35ug per lane.
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
WB, E
Primary Accession P20293
Reactivity Mouse
Host Rabbit
Clonality Polyclonal
Isotype Rabbit IgG
Calculated MW 47440 Da
Additional Information
Gene ID 20997
Other Names Brachyury protein, Protein T, T
Target/Specificity This Mouse T antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide between 408-442 amino acids from the C-terminal region of human Mouse T.
Dilution WB~~1:1000
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsMouse T Antibody (C-term) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name Tbxt {ECO:0000250|UniProtKB:O15178}
Function Involved in the transcriptional regulation of genes required for mesoderm formation and differentiation (PubMed:7588606). Binds to a palindromic T site 5'-TTCACACCTAGGTGTGAA-3' DNA sequence and activates gene transcription when bound to such a site (PubMed:8344258, PubMed:7588606).
Cellular Location Nucleus.
Research Areas
Citations (0)
citation

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Background

Involved in the transcriptional regulation of genes required for mesoderm formation and differentiation. Binds to a palindromic site (called T site) and activates gene transcription when bound to such a site.

References

Herrmann B.G.,et al.Nature 343:617-622(1990).
Kispert A.,et al.EMBO J. 12:3211-3220(1993).
Kispert A.,et al.EMBO J. 14:4763-4772(1995).

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$ 365.00
$ 140.00
Cat# AP20762c
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