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Smad3 Antibody (Center)
Purified Rabbit Polyclonal Antibody (Pab)
- SPECIFICATION
- CITATIONS
- PROTOCOLS
- BACKGROUND
Application 
| WB, E |
|---|---|
| Primary Accession | P84025 |
| Other Accession | P84023, P84022, Q8BUN5, P84024, P84025 |
| Reactivity | Rat |
| Predicted | Chicken, Human, Mouse, Pig |
| Host | Rabbit |
| Clonality | polyclonal |
| Isotype | Rabbit IgG |
| Other Names | Mothers against decapentaplegic homolog 3, MAD homolog 3, Mad3, Mothers against DPP homolog 3, mMad3, SMAD family member 3, SMAD 3, Smad3, Smad3, Madh3 |
|---|---|
| Target/Specificity | This Smad3 antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide between 157-191 amino acids from the Central region of rat Smad3. |
| Dilution | WB~~1:2000 E~~Use at an assay dependent concentration. |
| Format | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
| Precautions | Smad3 Antibody (Center) is for research use only and not for use in diagnostic or therapeutic procedures. |
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Provided below are standard protocols that you may find useful for product applications.
Background
Receptor-regulated SMAD (R-SMAD) that is an intracellular signal transducer and transcriptional modulator activated by TGF-beta (transforming growth factor) and activin type 1 receptor kinases. Binds the TRE element in the promoter region of many genes that are regulated by TGF-beta and, on formation of the SMAD3/SMAD4 complex, activates transcription. Also can form a SMAD3/SMAD4/JUN/FOS complex at the AP-1/SMAD site to regulate TGF-beta-mediated transcription. Has an inhibitory effect on wound healing probably by modulating both growth and migration of primary keratinocytes and by altering the TGF- mediated chemotaxis of monocytes. This effect on wound healing appears to be hormone-sensitive. Regulator of chondrogenesis and osteogenesis and inhibits early healing of bone fractures. Positively regulates PDPK1 kinase activity by stimulating its dissociation from the 14-3-3 protein YWHAQ which acts as a negative regulator (By similarity).
References
Kano K.,et al.J. Vet. Med. Sci. 61:213-219(1999).
Yang X.,et al.Submitted (JUL-1997) to the EMBL/GenBank/DDBJ databases.
Carninci P.,et al.Science 309:1559-1563(2005).
Liu X.,et al.Proc. Natl. Acad. Sci. U.S.A. 94:10669-10674(1997).
Ashcroft G.S.,et al.Nat. Cell Biol. 1:260-266(1999).
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