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GNG2 Antibody (N-Term)

Purified Rabbit Polyclonal Antibody (Pab)

     
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  • WB - GNG2 Antibody (N-Term) AP21903a
    All lanes : Anti-GNG2 Antibody (N-Term) at 1:8000 dilution Lane 1: human brain lysate Lane 2: mouse brain lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 8 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
    detail
  • IHC-P - GNG2 Antibody (N-Term) AP21903a
    AP21903a staining GNG2 in human brain tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde and blocked with 3% BSA for 0. 5 hour at room temperature; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody (1/25) for 1 hours at 37°C. A undiluted biotinylated goat polyvalent antibody was used as the secondary antibody.
    detail
  • SPECIFICATION
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
WB, IHC-P, E
Primary Accession P59768
Other Accession P63212, P63213, Q5R7U4
Reactivity Human, Mouse
Predicted Bovine
Host Rabbit
Clonality polyclonal
Isotype Rabbit IgG
Calculated MW 7850 Da
Antigen Region 19-52 aa
Additional Information
Gene ID 54331
Other Names Guanine nucleotide-binding protein G(I)/G(S)/G(O) subunit gamma-2, G gamma-I, GNG2
Target/Specificity This GNG2 antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide between 19-52 amino acids from of human GNG2.
Dilution WB~~1:8000
IHC-P~~1:25
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsGNG2 Antibody (N-Term) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name GNG2
Function Guanine nucleotide-binding proteins (G proteins) are involved as a modulator or transducer in various transmembrane signaling systems. The beta and gamma chains are required for the GTPase activity, for replacement of GDP by GTP, and for G protein-effector interaction (By similarity).
Cellular Location Cell membrane; Lipid-anchor; Cytoplasmic side
Tissue Location Expressed in fetal tissues, including testis, adrenal gland, brain, white blood cells and brain
Research Areas
Citations (0)
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Background

Guanine nucleotide-binding proteins (G proteins) are involved as a modulator or transducer in various transmembrane signaling systems. The beta and gamma chains are required for the GTPase activity, for replacement of GDP by GTP, and for G protein- effector interaction (By similarity).

References

Modarressi M.H.,et al.Biochem. Biophys. Res. Commun. 272:610-615(2000).
Puhl H.L. III,et al.Submitted (MAR-2002) to the EMBL/GenBank/DDBJ databases.
Bechtel S.,et al.BMC Genomics 8:399-399(2007).
Mural R.J.,et al.Submitted (SEP-2005) to the EMBL/GenBank/DDBJ databases.
Gauci S.,et al.Anal. Chem. 81:4493-4501(2009).

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$ 365.00
$ 140.00
Cat# AP21903a
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