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LARGE Antibody (Center)
Purified Rabbit Polyclonal Antibody (Pab)
- SPECIFICATION
- CITATIONS
- PROTOCOLS
- BACKGROUND
Application 
| WB, E |
|---|---|
| Primary Accession | O95461 |
| Other Accession | Q66PG4, Q66PG1, Q8N3Y3, Q66PG3, Q66PG2, Q9Z1M7 |
| Reactivity | Human |
| Predicted | Chicken, Zebrafish, Mouse |
| Host | Rabbit |
| Clonality | polyclonal |
| Isotype | Rabbit IgG |
| Calculated MW | 88066 Da |
| Gene ID | 9215 |
|---|---|
| Other Names | Glycosyltransferase-like protein LARGE1, 2.4.-.-, Acetylglucosaminyltransferase-like 1A, Xylosyltransferase LARGE, 2.4.2.-, Beta-1, 3-glucuronyltransferase LARGE, 2.4.1.-, LARGE, KIAA0609, LARGE1 |
| Target/Specificity | This LARGE antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide between 365-398 amino acids from the Central region of human LARGE. |
| Dilution | WB~~1:2000 |
| Format | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
| Precautions | LARGE Antibody (Center) is for research use only and not for use in diagnostic or therapeutic procedures. |
| Name | LARGE1 (HGNC:6511) |
|---|---|
| Synonyms | KIAA0609, LARGE |
| Function | Bifunctional glycosyltransferase with both alpha-1,3- xylosyltransferase and beta-1,3-glucuronyltransferase activities involved in the maturation of alpha-dystroglycan (DAG1) by glycosylation leading to DAG1 binding to laminin G-like domain- containing extracellular proteins with high affinity (PubMed:15661757, PubMed:15752776, PubMed:21987822, PubMed:22223806, PubMed:23125099, PubMed:25279697, PubMed:25279699). Elongates the glucuronyl-beta-1,4- xylose-beta disaccharide primer structure initiated by B4GAT1 by adding repeating units [-3-Xylose-alpha-1,3-GlcA-beta-1-] to produce a heteropolysaccharide (PubMed:22223806, PubMed:23125099, PubMed:25138275, PubMed:25279697, PubMed:25279699, PubMed:32975514). Requires the phosphorylation of core M3 (O-mannosyl trisaccharide) by POMK to elongate the glucuronyl-beta-1,4-xylose-beta disaccharide primer (PubMed:21987822). Plays a key role in skeletal muscle function and regeneration (By similarity). |
| Cellular Location | Golgi apparatus membrane; Single-pass type II membrane protein |
| Tissue Location | Ubiquitous. Highest expression in heart, brain and skeletal muscle. |
Thousands of laboratories across the world have published research that depended on the performance of antibodies from Abcepta to advance their research. Check out links to articles that cite our products in major peer-reviewed journals, organized by research category.
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Provided below are standard protocols that you may find useful for product applications.
Background
Bifunctional glycosyltransferase with both xylosyltransferase and beta-1,3-glucuronyltransferase activities involved in the biosynthesis of the phosphorylated O-mannosyl trisaccharide (N-acetylgalactosamine-beta-3-N-acetylglucosamine- beta-4-(phosphate-6-)mannose), a carbohydrate structure present in alpha-dystroglycan (DAG1) (PubMed:22223806). Phosphorylated O- mannosyl trisaccharid is required for binding laminin G-like domain-containing extracellular proteins with high affinity and plays a key role in skeletal muscle function and regeneration. LARGE elongates the glucuronyl-beta-1,4-xylose-beta disaccharide primer structure initiated by B3GNT1/B4GAT1 by adding repeating units [-3-Xylose-alpha-1,3-GlcA-beta-1-] to produce a heteropolysaccharide (PubMed:25279699).
References
Peyrard M.,et al.Proc. Natl. Acad. Sci. U.S.A. 96:598-603(1999).
Nagase T.,et al.DNA Res. 5:31-39(1998).
Collins J.E.,et al.Genome Biol. 5:R84.1-R84.11(2004).
Dunham I.,et al.Nature 402:489-495(1999).
Fujimura K.,et al.Biochem. Biophys. Res. Commun. 329:1162-1171(2005).
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