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CHD5 Antibody (N-Term)
Purified Rabbit Polyclonal Antibody (Pab)
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Application 
| WB, E |
|---|---|
| Primary Accession | Q8TDI0 |
| Reactivity | Human |
| Host | Rabbit |
| Clonality | polyclonal |
| Isotype | Rabbit IgG |
| Calculated MW | 223050 Da |
| Gene ID | 26038 |
|---|---|
| Other Names | Chromodomain-helicase-DNA-binding protein 5, CHD-5, 3.6.4.12, ATP-dependent helicase CHD5, CHD5 {ECO:0000312|EMBL:AAL98962.1}, KIAA0444 |
| Target/Specificity | This CHD5 antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide between 64-98 amino acids from human CHD5. |
| Dilution | WB~~1:2000 E~~Use at an assay dependent concentration. |
| Format | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
| Precautions | CHD5 Antibody (N-Term) is for research use only and not for use in diagnostic or therapeutic procedures. |
| Name | CHD5 {ECO:0000312|EMBL:AAL98962.1} |
|---|---|
| Synonyms | KIAA0444 |
| Function | ATP-dependent chromatin-remodeling factor that binds DNA through histones and regulates gene transcription. May specifically recognize and bind trimethylated 'Lys-27' (H3K27me3) and non-methylated 'Lys-4' of histone H3. Acts as a component of the histone deacetylase NuRD complex which participates in the remodeling of chromatin. Plays a role in the development of the nervous system by activating the expression of genes promoting neuron terminal differentiation. In parallel, it may also positively regulate the trimethylation of histone H3 at 'Lys-27' thereby specifically repressing genes that promote the differentiation into non-neuronal cell lineages. Regulates the expression of genes involved in cell proliferation and differentiation. Downstream activated genes may include CDKN2A that positively regulates the p53/TP53 pathway, which in turn, prevents cell proliferation. In spermatogenesis, it probably regulates histone hyperacetylation and the replacement of histones by transition proteins in chromatin, a crucial step in the condensation of spermatid chromatin and the production of functional spermatozoa. |
| Cellular Location | Nucleus. Chromosome {ECO:0000250|UniProtKB:A2A8L1} |
| Tissue Location | Preferentially expressed in total brain, fetal brain, and cerebellum. It is also moderately expressed in the adrenal gland and detected in testis. |
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Provided below are standard protocols that you may find useful for product applications.
Background
Chromatin-remodeling protein that binds DNA through histones and regulates gene transcription. May specifically recognize and bind trimethylated 'Lys-27' (H3K27me3) and non- methylated 'Lys-4' of histone H3. Plays a role in the development of the nervous system by activating the expression of genes promoting neuron terminal differentiation. In parallel, it may also positively regulate the trimethylation of histone H3 at 'Lys- 27' thereby specifically repressing genes that promote the differentiation into non-neuronal cell lineages. Tumor suppressor, it regulates the expression of genes involved in cell proliferation and differentiation. Downstream activated genes may include CDKN2A that positively regulates the p53/TP53 pathway, which in turn, prevents cell proliferation. In spermatogenesis, it probably regulates histone hyperacetylation and the replacement of histones by transition proteins in chromatin, a crucial step in the condensation of spermatid chromatin and the production of functional spermatozoa.
References
Thompson P.M.,et al.Oncogene 22:1002-1011(2003).
Gregory S.G.,et al.Nature 441:315-321(2006).
Bechtel S.,et al.BMC Genomics 8:399-399(2007).
Seki N.,et al.DNA Res. 4:345-349(1997).
Bagchi A.,et al.Cell 128:459-475(2007).
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