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RNF36 (TRIM69) Antibody (Center H215)
Purified Rabbit Polyclonal Antibody (Pab)
- SPECIFICATION
- CITATIONS
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- BACKGROUND
Application 
| WB, E |
|---|---|
| Primary Accession | Q86WT6 |
| Reactivity | Human |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | Rabbit IgG |
| Calculated MW | 57419 Da |
| Antigen Region | 200-231 aa |
| Gene ID | 140691 |
|---|---|
| Other Names | E3 ubiquitin-protein ligase TRIM69, 632-, RFP-like domain-containing protein trimless, RING finger protein 36, Tripartite motif-containing protein 69, TRIM69, RNF36 |
| Target/Specificity | This RNF36 (TRIM69) antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 200-231 amino acids from the Central region of human RNF36 (TRIM69). |
| Dilution | WB~~1:1000 |
| Format | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS. |
| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
| Precautions | RNF36 (TRIM69) Antibody (Center H215) is for research use only and not for use in diagnostic or therapeutic procedures. |
| Name | TRIM69 |
|---|---|
| Synonyms | RNF36 |
| Function | E3 ubiquitin ligase that plays an important role in antiviral immunity by restricting different viral infections including dengue virus or vesicular stomatitis indiana virus (PubMed:23131556, PubMed:30142214, PubMed:31375575, PubMed:31578292). Ubiquitinates viral proteins such as dengue virus NS3 thereby limiting infection (PubMed:30844644). In addition, acts as a key mediator of type I interferon induced microtubule stabilization by directly associating to microtubules independently of its E3 ligase activity (PubMed:36251989). Also plays a role in cataract formation together with TP53 (PubMed:30844644). Mechanistically, inhibits UVB-induced cell apoptosis and reactive oxygen species (ROS) production by inducing TP53 ubiquitination (PubMed:30844644). Regulates centrosome dynamics and mitotic progression by ubiquitinating STK3/MST2; leading to its redistribution to the perinuclear cytoskeleton and subsequent phosphorylation by PLK1 (PubMed:37739411). |
| Cellular Location | Cytoplasm. Nucleus. Nucleus speckle. Cytoplasm, cytoskeleton, microtubule organizing center, centrosome Note=Adopts a filamentous distribution in the cell cytoplasm where it strongly colocalizes with stable microtubules |
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Provided below are standard protocols that you may find useful for product applications.
Background
TRIM69 is a member of the RING-B-box-coiled-coil (RBCC) family with an N-terminal RING finger motif, a PRY domain and a C-terminal SPRY domain. The mouse ortholog of TRIM69 is specifically expressed in germ cells at the round spermatid stages during spermatogenesis and, when overexpressed, induces apoptosis.
References
Shyu,H.W., Exp. Cell Res. 287 (2), 301-313 (2003)
Shyu,H.W., Mech. Dev. 108 (1-2), 213-216 (2001)
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