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RNF36 (TRIM69) Antibody (Center K251)

Purified Rabbit Polyclonal Antibody (Pab)

     
  • WB - RNF36 (TRIM69) Antibody (Center K251) AP2525d
    Western blot analysis of anti-TRIM69 Antibody (Center K251) (RB13944) in HL60 cell line lysates (35ug/lane). TRIM69(arrow) was detected using the purified Pab.
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
WB, E
Primary Accession Q86WT6
Reactivity Human
Host Rabbit
Clonality Polyclonal
Isotype Rabbit IgG
Calculated MW 57419 Da
Antigen Region 336-365 aa
Additional Information
Gene ID 140691
Other Names E3 ubiquitin-protein ligase TRIM69, 632-, RFP-like domain-containing protein trimless, RING finger protein 36, Tripartite motif-containing protein 69, TRIM69, RNF36
Target/Specificity This RNF36 (TRIM69) antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 336-365 amino acids from the Central region of human RNF36 (TRIM69).
Dilution WB~~1:1000
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsRNF36 (TRIM69) Antibody (Center K251) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name TRIM69
Synonyms RNF36
Function E3 ubiquitin ligase that plays an important role in antiviral immunity by restricting different viral infections including dengue virus or vesicular stomatitis indiana virus (PubMed:23131556, PubMed:30142214, PubMed:31375575, PubMed:31578292). Ubiquitinates viral proteins such as dengue virus NS3 thereby limiting infection (PubMed:30844644). In addition, acts as a key mediator of type I interferon induced microtubule stabilization by directly associating to microtubules independently of its E3 ligase activity (PubMed:36251989). Plays also a role in cataract formation together with TP53 (PubMed:30844644). Mechanistically, inhibits UVB-induced cell apoptosis and reactive oxygen species (ROS) production by inducing TP53 ubiquitination (PubMed:30844644). Regulates centrosome dynamics and mitotic progression by ubiquitinating STK3/MST2; leading to its redistribution to the perinuclear cytoskeleton and subsequent phosphorylation by PLK1 (PubMed:37739411).
Cellular Location Cytoplasm. Nucleus. Nucleus speckle. Cytoplasm, cytoskeleton, microtubule organizing center, centrosome Note=Adopts a filamentous distribution in the cell cytoplasm where it strongly colocalizes with stable microtubules
Research Areas
Citations (0)
citation

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Background

TRIM69 is a member of the RING-B-box-coiled-coil (RBCC) family with an N-terminal RING finger motif, a PRY domain and a C-terminal SPRY domain. The mouse ortholog of TRIM69 is specifically expressed in germ cells at the round spermatid stages during spermatogenesis and, when overexpressed, induces apoptosis.

References

Shyu,H.W., Exp. Cell Res. 287 (2), 301-313 (2003)
Shyu,H.W., Mech. Dev. 108 (1-2), 213-216 (2001)

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$ 365.00
$ 140.00
Cat# AP2525d
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