Ihh Antibody (N-term)
Affinity Purified Rabbit Polyclonal Antibody (Pab)
- SPECIFICATION
- CITATIONS: 1
- PROTOCOLS
- BACKGROUND
Application
| WB, IHC-P, E |
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Primary Accession | Q80XI9 |
Reactivity | Human, Mouse |
Host | Rabbit |
Clonality | Polyclonal |
Isotype | Rabbit IgG |
Calculated MW | 49445 Da |
Antigen Region | 1-30 aa |
Gene ID | 16147 |
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Other Names | Indian hedgehog |
Target/Specificity | This Ihh antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 1-30 amino acids from the N-terminal region of human Ihh. |
Dilution | WB~~1:1000 IHC-P~~1:10~50 |
Format | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
Precautions | Ihh Antibody (N-term) is for research use only and not for use in diagnostic or therapeutic procedures. |
Name | Ihh {ECO:0000313|EMBL:AAH46984.1, ECO:0000313|MGI:MGI:96533} |
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Function | [Protein hedgehog N-product]: The dually lipidated hedgehog protein N-product is a morphogen which is essential for a variety of patterning events during development. |
Cellular Location | Endoplasmic reticulum membrane {ECO:0000256|ARBA:ARBA00004586}. Membrane {ECO:0000256|ARBA:ARBA00004635}; Lipid-anchor {ECO:0000256|ARBA:ARBA00004635}. Secreted {ECO:0000256|ARBA:ARBA00004613} [Protein hedgehog N-product]: Cell membrane {ECO:0000256|RuleBase:RU280812}; Lipid-anchor {ECO:0000256|RuleBase:RU280812} |
Provided below are standard protocols that you may find useful for product applications.
Background
Ihh (Indian hedgehog) is a critical and possibly direct regulator of joint development. In its absence, distribution and function of Gdf5-expressing interzone-associated cells are abnormal.
References
Kobayashi,T., Proc. Natl. Acad. Sci. U.S.A. 105 (6), 1949-1954 (2008) Gat-Yablonski,G.,J. Pediatr. Endocrinol. Metab. 20 (11), 1215-1222 (2007) Koyama,E., Ann. N. Y. Acad. Sci. 1116, 100-112 (2007)
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