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Arhgef9 Antibody (Center)

Affinity Purified Rabbit Polyclonal Antibody (Pab)

     
  • WB - Arhgef9 Antibody (Center) AP2713c
    All lanes : Anti-Collybistin(Arhgef9) (Center) at 1:1000 dilution Lane 1: rat brain lysate Lane 2: rat heart lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 58 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
    detail
  • IHC-P - Arhgef9 Antibody (Center) AP2713c
    Formalin-fixed and paraffin-embedded human brain tissue reacted with Arhgef9 Antibody (Center) (Cat.#AP2713c), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
    detail
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
IHC-P, WB, E
Primary Accession Q9QX73
Other Accession Q3UTH8, O43307, Q58DL7
Reactivity Rat
Predicted Bovine, Human, Mouse
Host Rabbit
Clonality Polyclonal
Isotype Rabbit IgG
Calculated MW 58157 Da
Antigen Region 274-303 aa
Additional Information
Gene ID 66013
Other Names Rho guanine nucleotide exchange factor 9, Collybistin, Rac/Cdc42 guanine nucleotide exchange factor 9, Arhgef9
Target/Specificity This Arhgef9 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 274-303 amino acids from the Central region of human Arhgef9.
Dilution WB~~1:1000
IHC-P~~1:10~50
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsArhgef9 Antibody (Center) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name Arhgef9
Function Acts as a guanine nucleotide exchange factor (GEF) for CDC42. Promotes formation of GPHN clusters.
Cellular Location Cytoplasm. Postsynaptic density {ECO:0000250|UniProtKB:Q3UTH8}
Tissue Location Detected in brain, throughout the gray matter. Detected at low levels in heart and skeletal muscle
Research Areas
Citations (0)
citation

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Background

ARHGEF9 belongs to a family of Rho-like GTPases that act as molecular switches by cycling from the active GTP-bound state to the inactive GDP-bound state. These proteins are key regulators of the actin cytoskeleton and are involved in cell signaling.

References

Xiang,S., J. Mol. Biol. 359 (1), 35-46 (2006)
Harvey,K., J. Neurosci. 24 (25), 5816-5826 (2004)
Grosskreutz,Y., Biol. Chem. 382 (10), 1455-1462 (2001)
Kins,S., Nat. Neurosci. 3 (1), 22-29 (2000)

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$ 365.00
$ 140.00
Cat# AP2713c
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