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STIP1 Antibody (Center)

Purified Rabbit Polyclonal Antibody (Pab)

     
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  • WB - STIP1 Antibody (Center) AP2817c
    STIP1 Antibody (Center) (Cat.# AP2817c) western blot analysis in A431,A549 cell line lysates (35ug/lane).This demonstrates the STIP1 antibody detected the STIP1 protein (arrow).
    detail
  • IHC-P - STIP1 Antibody (Center) AP2817c
    Formalin-fixed and paraffin-embedded human testis tissue reacted with STIP1 Antibody (Center) (Cat.# AP2817c), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
    detail
  • FC - STIP1 Antibody (Center) AP2817c
    STIP1 Antibody (Center) (Cat. #AP2817c) flow cytometry analysis of Jurkat cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
    detail
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
IHC-P, WB, FC, E
Primary Accession P31948
Other Accession O35814, Q60864, O54981, Q3ZBZ8, Q7ZWU1
Reactivity Human
Predicted Bovine, Hamster, Mouse, Rat, Xenopus
Host Rabbit
Clonality Polyclonal
Isotype Rabbit IgG
Calculated MW 62639 Da
Antigen Region 269-297 aa
Additional Information
Gene ID 10963
Other Names Stress-induced-phosphoprotein 1, STI1, Hsc70/Hsp90-organizing protein, Hop, Renal carcinoma antigen NY-REN-11, Transformation-sensitive protein IEF SSP 3521, STIP1
Target/Specificity This STIP1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 269-297 amino acids from the Central region of human STIP1.
Dilution WB~~1:1000
IHC-P~~1:50~100
FC~~1:10~50
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsSTIP1 Antibody (Center) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name STIP1 (HGNC:11387)
Function Acts as a co-chaperone for HSP90AA1 (PubMed:27353360). Mediates the association of the molecular chaperones HSPA8/HSC70 and HSP90 (By similarity).
Cellular Location Cytoplasm {ECO:0000250|UniProtKB:Q60864}. Nucleus {ECO:0000250|UniProtKB:Q60864}. Dynein axonemal particle {ECO:0000250|UniProtKB:Q7ZWU1}
Research Areas
Citations (0)
citation

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Background

STIP1 mediates the association of the molecular chaperones HSC70 and HSP90 (HSPCA and HSPCB).

References

Onuoha,S.C., J. Mol. Biol. 379 (4), 732-744 (2008) Erlich,R.B., Glia 55 (16), 1690-1698 (2007)

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$ 365.00
$ 140.00
Cat# AP2817c
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