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POLG2 Antibody (C-term)
Purified Rabbit Polyclonal Antibody (Pab)
- SPECIFICATION
- CITATIONS: 1
- PROTOCOLS
- BACKGROUND
Application 
| FC, WB, IHC-P, E |
|---|---|
| Primary Accession | Q9UHN1 |
| Reactivity | Human |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | Rabbit IgG |
| Calculated MW | 54911 Da |
| Antigen Region | 348-376 aa |
| Gene ID | 11232 |
|---|---|
| Other Names | DNA polymerase subunit gamma-2, mitochondrial, DNA polymerase gamma accessory 55 kDa subunit, p55, Mitochondrial DNA polymerase accessory subunit, MtPolB, PolG-beta, POLG2, MTPOLB |
| Target/Specificity | This POLG2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 348-376 amino acids from the C-terminal region of human POLG2. |
| Dilution | WB~~1:1000 IHC-P~~1:50~100 FC~~1:10~50 |
| Format | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS. |
| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
| Precautions | POLG2 Antibody (C-term) is for research use only and not for use in diagnostic or therapeutic procedures. |
| Name | POLG2 {ECO:0000303|PubMed:30157269, ECO:0000312|HGNC:HGNC:9180} |
|---|---|
| Function | Accessory subunit of DNA polymerase gamma solely responsible for replication of mitochondrial DNA (mtDNA). Acts as an allosteric regulator of the holoenzyme activities. Enhances the polymerase activity and the processivity of POLG by increasing its interactions with the DNA template. Suppresses POLG exonucleolytic proofreading especially toward homopolymeric templates bearing mismatched termini. Binds to single-stranded DNA. |
| Cellular Location | Mitochondrion {ECO:0000250|UniProtKB:P54098}. Mitochondrion matrix, mitochondrion nucleoid {ECO:0000250|UniProtKB:P54098} |
Provided below are standard protocols that you may find useful for product applications.
Background
The accuracy of mitochondrial DNA (mtDNA) replication depends on the coordinated action of many nuclear-encoded proteins and on the correct balance of nucleotides within the mitochondrial matrix. mtDNA is replicated by DNA polymerase gamma, which is composed of a 140-kD catalytic subunit(POLG1) and a 55-kD accessory subunit (POLG2).
References
Longley,M.J., Am. J. Hum. Genet. 78 (6), 1026-1034 (2006)
Carrodeguas,J.A., J. Biol. Chem. 277 (51), 50008-50014 (2002)
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