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p47 phox Antibody (Ab-304)
Purified Rabbit Polyclonal Antibody (Pab)
- SPECIFICATION
- CITATIONS
- PROTOCOLS
- BACKGROUND
Application 
| WB, IF |
|---|---|
| Primary Accession | P14598 |
| Reactivity | Human |
| Host | Rabbit |
| Clonality | Polyclonal |
| Calculated MW | 45,31 KDa |
| Antigen Region | 295-330 aa |
| Gene ID | 653361 |
|---|---|
| Other Names | Neutrophil cytosol factor 1, NCF-1, 47 kDa autosomal chronic granulomatous disease protein, 47 kDa neutrophil oxidase factor, NCF-47K, Neutrophil NADPH oxidase factor 1, Nox organizer 2, Nox-organizing protein 2, SH3 and PX domain-containing protein 1A, p47-phox, NCF1, NOXO2, SH3PXD1A |
| Dilution | IF~~1:100 WB~~ 1:250-1:1000 |
| Format | Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.09% (W/V) sodium azide and 50% glycerol. |
| Storage Conditions | -20℃ |
| Name | NCF1 (HGNC:7660) |
|---|---|
| Synonyms | NOXO2, SH3PXD1A |
| Function | Subunit of the phagocyte NADPH oxidase complex that mediates the transfer of electrons from cytosolic NADPH to O2 to produce the superoxide anion (O2(-)) (PubMed:2547247, PubMed:2550933, PubMed:38355798). In the activated complex, electrons are first transferred from NADPH to flavin adenine dinucleotide (FAD) and subsequently transferred via two heme molecules to molecular oxygen, producing superoxide through an outer-sphere reaction (PubMed:38355798). Activation of the NADPH oxidase complex is initiated by the assembly of cytosolic subunits of the NADPH oxidase complex with the core NADPH oxidase complex to form a complex at the plasma membrane or phagosomal membrane (PubMed:38355798). This activation process is initiated by phosphorylation dependent binding of the cytosolic NCF1/p47-phox subunit to the C-terminus of CYBA/p22-phox (PubMed:12732142, PubMed:19801500). |
| Cellular Location | Cytoplasm, cytosol. Membrane; Peripheral membrane protein; Cytoplasmic side |
| Tissue Location | Detected in peripheral blood monocytes and neutrophils (at protein level). |
Thousands of laboratories across the world have published research that depended on the performance of antibodies from Abcepta to advance their research. Check out links to articles that cite our products in major peer-reviewed journals, organized by research category.
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Provided below are standard protocols that you may find useful for product applications.
Background
NCF2, NCF1, and a membrane bound cytochrome b558 are required for activation of the latent NADPH oxidase (necessary for superoxide production).
References
Volpp B.D.,et al.Proc. Natl. Acad. Sci. U.S.A. 86:7195-7199(1989).
Volpp B.D.,et al.Proc. Natl. Acad. Sci. U.S.A. 86:9563-9563(1989).
Lomax K.J.,et al.Science 245:409-412(1989).
Rodaway A.R.F.,et al.Mol. Cell. Biol. 10:5388-5396(1990).
Gorlach A.,et al.J. Clin. Invest. 100:1907-1918(1997).
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