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Anti-Collagen 20 alpha 1 Antibody

     
  • WB - Anti-Collagen 20 alpha 1 Antibody AP53740
    Western blot analysis of Collagen 20 alpha 1 expression in HT29 (A), NCIH292 (B) whole cell lysates.
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  • IH - Anti-Collagen 20 alpha 1 Antibody AP53740
    Immunohistochemical analysis of Collagen 20 alpha 1 staining in human brain formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
    detail
  • IF - Anti-Collagen 20 alpha 1 Antibody AP53740
    Immunofluorescent analysis of Collagen 20 alpha 1 staining in HT29 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
WB, IF, IH
Primary Accession Q9P218
Reactivity Human
Host Rabbit
Clonality Polyclonal
Calculated MW 135830 Da
Additional Information
Gene ID 57642
Other Names KIAA1510; Collagen alpha-1(XX) chain
Target/Specificity Recognizes endogenous levels of Collagen 20 alpha 1 protein.
Dilution WB~~1/500 - 1/1000
IH~~1/50 - 1/200
IF~~1/50 - 1/200
Format Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.09% (W/V) sodium azide.
StorageStore at -20 °C.Stable for 12 months from date of receipt
Protein Information
Name COL20A1
Synonyms KIAA1510
Function Probable collagen protein.
Cellular Location Secreted, extracellular space.
Tissue Location High expression in heart, lung, liver, skeletal muscle, kidney, pancreas, spleen, testis, ovary, subthalamic nucleus and fetal liver. Weak expression in other tissues tested
Research Areas
Citations (0)
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Background

Rabbit polyclonal antibody to Collagen 20 alpha 1

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$ 350.00
Cat# AP53740
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