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Anti-TLE2 Antibody

     
  • WB - Anti-TLE2 Antibody AP53781
    Western blot analysis of TLE2 expression in HeLa (A), Jurkat (B) whole cell lysates.
    detail
  • IH - Anti-TLE2 Antibody AP53781
    Immunohistochemical analysis of TLE2 staining in human lung cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
    detail
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
WB, IHC
Primary Accession Q04725
Reactivity Human
Host Rabbit
Clonality Polyclonal
Calculated MW 79841 Da
Additional Information
Gene ID 7089
Other Names Transducin-like enhancer protein 2; Enhancer of split groucho-like protein 2; ESG2
Target/Specificity Recognizes endogenous levels of TLE2 protein.
Dilution WB~~1/500 - 1/1000
IH~~1/50 - 1/200
Format Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.09% (W/V) sodium azide.
StorageStore at -20 °C.Stable for 12 months from date of receipt
Protein Information
Name TLE2
Function Transcriptional corepressor that binds to a number of transcription factors. Inhibits the transcriptional activation mediated by CTNNB1 and TCF family members in Wnt signaling. The effects of full- length TLE family members may be modulated by association with dominant-negative AES (By similarity).
Cellular Location Nucleus.
Tissue Location In all tissues examined, mostly in heart, brain, and muscle
Citations (0)
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Background

Rabbit polyclonal antibody to TLE2

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$ 350.00
Cat# AP53781
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