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Anti-Contactin 4 Antibody

     
  • WB - Anti-Contactin 4 Antibody AP54099
    Western blot analysis of Contactin 4 expression in mouse brain (A), rat brain (B) whole cell lysates.
    detail
  • IF - Anti-Contactin 4 Antibody AP54099
    Immunofluorescent analysis of Contactin 4 staining in A549 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a hidified chamber. Cells were washed with PBST and incubated with a Alexa Fluor 594-conjugated secondary antibody (red) in PBS at room temperature in the dark.
    detail
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
WB, IF
Primary Accession Q8IWV2
Reactivity Human, Mouse, Rat
Host Rabbit
Clonality Polyclonal
Calculated MW 113454 Da
Additional Information
Gene ID 152330
Other Names Contactin-4; Brain-derived immunoglobulin superfamily protein 2; BIG-2
Target/Specificity Recognizes endogenous levels of Contactin 4 protein.
Dilution WB~~1/500 - 1/1000
IF~~1/50 - 1/200
Format Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.09% (W/V) sodium azide.
StorageStore at -20 °C.Stable for 12 months from date of receipt
Protein Information
Name CNTN4
Function Contactins mediate cell surface interactions during nervous system development. Has some neurite outgrowth-promoting activity. May be involved in synaptogenesis.
Cellular Location Cell membrane; Lipid-anchor, GPI-anchor. Secreted
Tissue Location Mainly expressed in brain. Highly expressed in cerebellum and weakly expressed in corpus callosum, caudate nucleus, amygdala and spinal cord. Also expressed in testis, pancreas, thyroid, uterus, small intestine and kidney. Not expressed in skeletal muscle Isoform 2 is weakly expressed in cerebral cortex
Research Areas
Citations (0)
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Background

Rabbit polyclonal antibody to Contactin 4

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$ 350.00
Cat# AP54099
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