SLC9A9 Antibody (C-term)
Affinity Purified Rabbit Polyclonal Antibody (Pab)
- SPECIFICATION
- CITATIONS
- PROTOCOLS
- BACKGROUND
Application
| FC, IHC-P, WB, E |
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Primary Accession | Q8IVB4 |
Other Accession | Q8BZ00, NP_775924.1 |
Reactivity | Human, Mouse |
Host | Rabbit |
Clonality | Polyclonal |
Isotype | Rabbit IgG |
Calculated MW | 72565 Da |
Antigen Region | 551-579 aa |
Gene ID | 285195 |
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Other Names | Sodium/hydrogen exchanger 9, Na(+)/H(+) exchanger 9, NHE-9, Solute carrier family 9 member 9, SLC9A9, NHE9 |
Target/Specificity | This SLC9A9 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 551-579 amino acids from the C-terminal region of human SLC9A9. |
Dilution | WB~~1:1000 IHC-P~~1:50~100 FC~~1:10~50 |
Format | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
Precautions | SLC9A9 Antibody (C-term) is for research use only and not for use in diagnostic or therapeutic procedures. |
Name | SLC9A9 (HGNC:20653) |
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Synonyms | NHE9 |
Function | Endosomal Na(+), K(+)/H(+) antiporter. Mediates the electroneutral exchange of endosomal luminal H(+) for a cytosolic Na(+) or K(+) (Probable). By facilitating proton efflux, SLC9A9 counteracts the acidity generated by vacuolar (V)-ATPase, thereby limiting luminal acidification. Regulates organellar pH and consequently, e.g., endosome maturation and endocytic trafficking of plasma membrane receptors and neurotransporters (PubMed:28130443, PubMed:15522866, PubMed:24065030). Promotes the recycling of transferrin receptors back to the cell surface to facilitate additional iron uptake in the brain (PubMed:28130443). Regulates synaptic transmission by regulating the luminal pH of axonal endosomes (By similarity). Regulates phagosome lumenal pH, thus affecting phagosome maturation, and consequently, microbicidal activity in macrophages (By similarity). Can also be active at the cell surface of specialized cells, e.g., in the inner ear hair bundles uses the high K(+) of the endolymph to regulate intracelular pH (By similarity). |
Cellular Location | Late endosome membrane; Multi-pass membrane protein {ECO:0000250|UniProtKB:F7B113}. Early endosome membrane; Multi-pass membrane protein {ECO:0000250|UniProtKB:F7B113}. Recycling endosome membrane; Multi-pass membrane protein {ECO:0000250|UniProtKB:F7B113}. Cell membrane {ECO:0000250|UniProtKB:Q8BZ00}; Multi-pass membrane protein {ECO:0000250|UniProtKB:F7B113}. Cytoplasmic vesicle, phagosome membrane {ECO:0000250|UniProtKB:Q8BZ00}; Multi-pass membrane protein {ECO:0000250|UniProtKB:F7B113}. Note=Localized to the plasma membrane in inner ear hair cell bundle. {ECO:0000250|UniProtKB:Q8BZ00} |
Tissue Location | Ubiquitously expressed in all tissues tested. Expressed at highest levels in heart and skeletal muscle, followed by placenta, kidney, and liver. Expressed in the brain, in the medulla and spinal cord. |
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Provided below are standard protocols that you may find useful for product applications.
Background
This gene encodes a sodium hydrogen exchanger that is a member of the solute carrier 9 protein family. The encoded protein localizes the to the late recycling endosomes and may play an important role in maintaining cation homeostasis. Defects in this gene are associated with attention-deficit/hyperactivity disorder.
References
Markunas, C.A., et al. Psychiatr. Genet. (2009) In press :
Vink, J.M., et al. Am. J. Hum. Genet. 84(3):367-379(2009)
Lasky-Su, J., et al. Am. J. Med. Genet. B Neuropsychiatr. Genet. 147B (8), 1355-1358 (2008) :
Lasky-Su, J., et al. Am. J. Med. Genet. B Neuropsychiatr. Genet. 147B (8), 1345-1354 (2008) :
Ng, D., et al. Int. J. Cancer 123(7):1610-1615(2008)
Levy, D., et al. BMC Med. Genet. 8 SUPPL 1, S3 (2007) :
Nakamura, N., et al. J. Biol. Chem. 280(2):1561-1572(2005)
de Silva, M.G., et al. J. Med. Genet. 40(10):733-740(2003)
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