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Synaptotagmin XII Polyclonal Antibody

Purified Rabbit Polyclonal Antibody (Pab)

     
  • FC - Synaptotagmin XII Polyclonal Antibody AP54487
    Blank control:Hela.
    Primary Antibody (green line): Rabbit Anti- Synaptotagmin XII antibody (bs-11398R)
    Dilution: 2 µg /10^6 cells;
    Isotype Control Antibody (orange line): Rabbit IgG .
    Secondary Antibody : Goat anti-rabbit IgG-PE
    Dilution: 1 µg /test.
    Protocol
    The cells were incubated in 5%BSA to block non-specific protein-protein interactions for 30 min at room temperature .Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.
    detail
  • WB - Synaptotagmin XII Polyclonal Antibody AP54487
    Sample:
    U-2OS(Human) Cell Lysate at 30 ug
    Primary: Anti- Synaptotagmin XII (bs-11398R) at 1/1000 dilution
    Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
    Predicted band size: 47 kD
    Observed band size: 48 kD
    detail
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
WB, IHC-P, IHC-F, IF, ICC
Primary Accession Q8IV01
Reactivity Rat, Pig, Dog
Host Rabbit
Clonality Polyclonal
Calculated MW 46537 Da
Additional Information
Gene ID 91683
Other Names Synaptotagmin-12, SYT12
Dilution WB=1:500-2000,Elisa=1:500-1000,IHC-P=1:100-500,IHC-F=1:100-500,Flow Cyt=2ug/Test,IF=1:100-500,ICC=1:100-500,
Format0.01M TBS(pH7.4), 0.09% (W/V) sodium azide and 50% Glyce
StorageStore at -20 ℃ for one year. Avoid repeated freeze/thaw cycles. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 ℃.
Protein Information
Name SYT12
Function Synaptic vesicle phosphoprotein that enhances spontaneous neurotransmitter release but does not effect induced neurotransmitter release (By similarity). Unlike other synaptotagmins, it does not bind Ca(2+) or phospholipids (By similarity). Essential for mossy-fiber long-term potentiation in the hippocampus (By similarity).
Cellular Location Cytoplasmic vesicle, secretory vesicle, synaptic vesicle membrane {ECO:0000250|UniProtKB:P97610}; Single-pass membrane protein
Research Areas
Citations (0)
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$ 350.00
Cat# AP54487
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