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Progestin Receptor Beta Polyclonal Antibody

Purified Rabbit Polyclonal Antibody (Pab)

     
  • WB - Progestin Receptor Beta Polyclonal Antibody AP54490
    Sample:
    Lane 1: Hippocampus (Mouse) Lysate at 40 ug
    Lane 2: Medulld (Mouse) Lysate at 40 ug
    Lane 3: Hippocampus (Rat) Lysate at 40 ug
    Lane 4: Medulld (Rat) Lysate at 40 ug
    Primary:
    Anti-Progestin Receptor Beta (bs-11410R) at 1/1000 dilution
    Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
    Predicted band size: 42 kD
    Observed band size: 46 kD
    detail
  • IHC-P - Progestin Receptor Beta Polyclonal Antibody AP54490
    Paraformaldehyde-fixed, paraffin embedded (Dog skin tumor); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Progestin Receptor Beta) Polyclonal Antibody, Unconjugated (bs-11410R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
    detail
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
WB, IHC-P, IHC-F, IF, ICC
Primary Accession Q8TEZ7
Reactivity Rat, Pig, Dog, Bovine
Host Rabbit
Clonality Polyclonal
Calculated MW 40464 Da
Additional Information
Gene ID 85315
Other Names Membrane progestin receptor beta, mPR beta, Lysosomal membrane protein in brain 1, Membrane progesterone P4 receptor beta, Membrane progesterone receptor beta, Progesterone and adipoQ receptor family member 8, Progestin and adipoQ receptor family member 8, Progestin and adipoQ receptor family member VIII, PAQR8 (HGNC:15708)
Dilution WB=1:500-2000,Elisa=1:500-1000,IHC-P=1:100-500,IHC-F=1:100-500,IF=1:100-500,ICC=1:100-500,
Format0.01M TBS(pH7.4), 0.09% (W/V) sodium azide and 50% Glyce
StorageStore at -20 ℃ for one year. Avoid repeated freeze/thaw cycles. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 ℃.
Protein Information
Name PAQR8 (HGNC:15708)
Function Plasma membrane progesterone (P4) receptor coupled to G proteins (PubMed:23763432). Seems to act through a G(i) mediated pathway (PubMed:23763432). May be involved in oocyte maturation (By similarity). Also binds dehydroepiandrosterone (DHEA), pregnanolone, pregnenolone and allopregnanolone (PubMed:23161870).
Cellular Location Cell membrane; Multi-pass membrane protein. Note=Colocalizes with a lysosomal protein CTSD/cathepsin D.
Tissue Location Highly expressed in the hypothalamus (PubMed:23161870). Also expressed in spinal cord, kidney and testis
Research Areas
Citations (0)
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$ 350.00
Cat# AP54490
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