Register or Login
All
  • All
  • Uniprot Id
  • Catalog #
  • Peptide Sequence
COVID19
>   home   >   Products   >   Primary Antibodies   >   Signal Transduction   >   PDE7A Polyclonal Antibody   

PDE7A Polyclonal Antibody

Purified Rabbit Polyclonal Antibody (Pab)

     
  • FC - PDE7A Polyclonal Antibody AP54552
    Blank control(blue): RSC96 cells(fixed with 2% paraformaldehyde (10 min) , then permeabilized with 90% ice-cold methanol for 30 min on ice).
    Primary Antibody:Rabbit Anti- PDE7A antibody(bs-11575R), Dilution: 1 µg in 100 µL 1X PBS containing 0.5% BSA;
    Isotype Control Antibody: Rabbit IgG(orange) ,used under the same conditions );
    Secondary Antibody: Goat anti-rabbit IgG-PE(white blue), Dilution: 1:200 in 1 X PBS containing 0.5% BSA.
    detail
  • IHC-P - PDE7A Polyclonal Antibody AP54552
    Paraformaldehyde-fixed, paraffin embedded (rat heart); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (PDE7A) Polyclonal Antibody, Unconjugated (bs-11575R) at 1:500 overnight at 4°C, followed by a conjugated secondary (sp-0023) for 20 minutes and DAB staining.
    detail
  • WB - PDE7A Polyclonal Antibody AP54552
    Sample:Brain (Mouse)Lysate at 40 ug
    Primary: Anti-PDE7A(bs-11575R)at 1/300 dilution
    Secondary: IRDye800CW Goat Anti-RabbitIgG at 1/20000 dilution
    Predicted band size: 55kD
    Observed band size: 55kD
    detail
  • WB - PDE7A Polyclonal Antibody AP54552
    Sample: Muscle (Mouse)Lysate at 40 ug
    Primary: Anti-PDE7A(bs-11575R)at 1/300 dilution
    Secondary: IRDye800CW Goat Anti-RabbitIgG at 1/20000 dilution
    Predicted band size: 55kD
    Observed band size: 55kD
    detail
  • WB - PDE7A Polyclonal Antibody AP54552
    Sample: Hela Cell (Human) Lysate at 30 ug
    Primary: Anti-PDE7A (bs-11575R) at 1/300 dilution
    Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
    Predicted band size: 55kD
    Observed band size: 55kD
    detail
  • WB - PDE7A Polyclonal Antibody AP54552
    Sample: Lymph node (Mouse) Lysate at 40 ug
    Primary: Anti-PDE7A (bs-11575R) at 1/300 dilution
    Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
    Predicted band size: 55kD
    Observed band size: 55kD
    detail
  • SPECIFICATION
  • CITATIONS
  • PROTOCOLS
  • BACKGROUND
  • detail
Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
WB, IHC-P, IHC-F, IF, ICC
Primary Accession Q13946
Reactivity Rat, Pig, Dog, Cow
Host Rabbit
Clonality Polyclonal
Calculated MW 55505 Da
Additional Information
Gene ID 5150
Other Names High affinity cAMP-specific 3', 5'-cyclic phosphodiesterase 7A, 3.1.4.53, HCP1, TM22, PDE7A
Dilution WB=1:500-2000,Elisa=1:500-1000,IHC-P=1:100-500,IHC-F=1:100-500,Flow Cyt=1 µg/Test,IF=1:100-500,ICC=1:100-500,
Format0.01M TBS(pH7.4), 0.09% (W/V) sodium azide and 50% Glyce
StorageStore at -20 ℃ for one year. Avoid repeated freeze/thaw cycles. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 ℃.
Protein Information
Name PDE7A {ECO:0000303|PubMed:9195912, ECO:0000312|HGNC:HGNC:8791}
Function Hydrolyzes the second messenger cAMP, which is a key regulator of many important physiological processes (PubMed:8389765, PubMed:9195912, PubMed:19350606). May have a role in muscle signal transduction (PubMed:9195912).
Cellular Location [Isoform PDE7A1]: Cytoplasm, cytosol. Note=PDE7A1 (57 kDa) is located mostly to soluble cellular fractions.
Tissue Location [Isoform PDE7A1]: Found at high levels in skeletal muscle and at low levels in a variety of tissues including brain and heart (PubMed:9195912). It is expressed as well in two T-cell lines (PubMed:9195912).
Research Areas
Citations (0)
citation

Thousands of laboratories across the world have published research that depended on the performance of antibodies from Abcepta to advance their research. Check out links to articles that cite our products in major peer-reviewed journals, organized by research category.

Submit your citation using an Abcepta antibody to
info@abcepta.com, and receive a free "I Love Antibodies" mug.
FeedBack
Abcepta welcomes feedback from its customers.

If you have used an Abcepta product and would like to share how it has performed, please click on the "Submit Review" button and provide the requested information. Our staff will examine and post your review and contact you if needed.

If you have any additional inquiries please email technical services at tech@abcepta.com.

$ 350.00
Cat# AP54552
Size:
Quantity:
Availability: 2 weeks
Bulk Size

Ordering Information

United States
AlbaniaAustraliaAustriaBelgiumBosnia & HerzegovinaBrazilBulgariaCanadaCentral AmericaChinaCroatiaCyprusCzech RepublicDenmarkEstoniaFinlandFranceGermanyGreeceHong KongHungaryIcelandIndiaIndonesiaIrelandIsraelItalyJapanLatviaLithuaniaLuxembourgMacedoniaMalaysiaMaltaNetherlandsNew ZealandNorwayPakistanPolandPortugalRomaniaSerbiaSingaporeSlovakiaSloveniaSouth AfricaSouth KoreaSpainSwedenSwitzerlandTaiwanTurkeyUnited KingdomUnited StatesVietnamWorldwideOthers
Abcepta, Inc.
(888) 735-7227 / (858) 622-0099
(858) 622-0609
USA Headquarters
(888) 735-7227 / (858) 622-0099 or (858) 875-1900

Shipping Information

Domestic orders (in stock items)
Shipped out the same day. Orders placed after 1 PM (PST) will ship out the next business day.
International orders
Contact your local distributors
Terms & Conditions
"